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Comparing the Primary and Recall Immune Response Induced by a New EV71 Vaccine Using Systems Biology Approaches
Three inactivated EV71 whole-virus vaccines have completed Phase III clinical trials in mainland China, with high efficacy, satisfactory safety, and sustained immunogenicity. However, the molecular mechanisms how this new vaccine elicit potent immune response remain poorly understood. To characteriz...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4605509/ https://www.ncbi.nlm.nih.gov/pubmed/26465882 http://dx.doi.org/10.1371/journal.pone.0140515 |
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author | Shao, Jie Zhang, Junnan Wu, Xing Mao, Qunying Chen, Pan Zhu, Fengcai Xu, Miao Kong, Wei Liang, Zhenglun Wang, Junzhi |
author_facet | Shao, Jie Zhang, Junnan Wu, Xing Mao, Qunying Chen, Pan Zhu, Fengcai Xu, Miao Kong, Wei Liang, Zhenglun Wang, Junzhi |
author_sort | Shao, Jie |
collection | PubMed |
description | Three inactivated EV71 whole-virus vaccines have completed Phase III clinical trials in mainland China, with high efficacy, satisfactory safety, and sustained immunogenicity. However, the molecular mechanisms how this new vaccine elicit potent immune response remain poorly understood. To characterize the primary and recall responses to EV71 vaccines, PBMC from 19 recipients before and after vaccination with EV71 vaccine are collected and their gene expression signatures after stimulation with EV71 antigen were compared. The results showed that primary and recall response to EV71 antigen have both activated an IRF7 regulating type I interferon and antiviral immune response network. However, up-regulated genes involved in T cell activation regulated by IRF1, inflammatory response, B-cell activation and humoral immune response were only observed in recall response. The specific secretion of IL-10 in primary response and IL-2,IP-10,CCL14a, CCL21 in recall response was consistent with the activation of immune response process found in genes. Furthermore, the expression of MX1 and secretion of IP-10 in recall response were strongly correlated with NTAb level at 180d after vaccination (r = 0.81 and 0.99). In summary, inflammatory response, adaptive immune response and a stronger antiviral response were indentified in recall response. |
format | Online Article Text |
id | pubmed-4605509 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-46055092015-10-29 Comparing the Primary and Recall Immune Response Induced by a New EV71 Vaccine Using Systems Biology Approaches Shao, Jie Zhang, Junnan Wu, Xing Mao, Qunying Chen, Pan Zhu, Fengcai Xu, Miao Kong, Wei Liang, Zhenglun Wang, Junzhi PLoS One Research Article Three inactivated EV71 whole-virus vaccines have completed Phase III clinical trials in mainland China, with high efficacy, satisfactory safety, and sustained immunogenicity. However, the molecular mechanisms how this new vaccine elicit potent immune response remain poorly understood. To characterize the primary and recall responses to EV71 vaccines, PBMC from 19 recipients before and after vaccination with EV71 vaccine are collected and their gene expression signatures after stimulation with EV71 antigen were compared. The results showed that primary and recall response to EV71 antigen have both activated an IRF7 regulating type I interferon and antiviral immune response network. However, up-regulated genes involved in T cell activation regulated by IRF1, inflammatory response, B-cell activation and humoral immune response were only observed in recall response. The specific secretion of IL-10 in primary response and IL-2,IP-10,CCL14a, CCL21 in recall response was consistent with the activation of immune response process found in genes. Furthermore, the expression of MX1 and secretion of IP-10 in recall response were strongly correlated with NTAb level at 180d after vaccination (r = 0.81 and 0.99). In summary, inflammatory response, adaptive immune response and a stronger antiviral response were indentified in recall response. Public Library of Science 2015-10-14 /pmc/articles/PMC4605509/ /pubmed/26465882 http://dx.doi.org/10.1371/journal.pone.0140515 Text en © 2015 Shao et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Shao, Jie Zhang, Junnan Wu, Xing Mao, Qunying Chen, Pan Zhu, Fengcai Xu, Miao Kong, Wei Liang, Zhenglun Wang, Junzhi Comparing the Primary and Recall Immune Response Induced by a New EV71 Vaccine Using Systems Biology Approaches |
title | Comparing the Primary and Recall Immune Response Induced by a New EV71 Vaccine Using Systems Biology Approaches |
title_full | Comparing the Primary and Recall Immune Response Induced by a New EV71 Vaccine Using Systems Biology Approaches |
title_fullStr | Comparing the Primary and Recall Immune Response Induced by a New EV71 Vaccine Using Systems Biology Approaches |
title_full_unstemmed | Comparing the Primary and Recall Immune Response Induced by a New EV71 Vaccine Using Systems Biology Approaches |
title_short | Comparing the Primary and Recall Immune Response Induced by a New EV71 Vaccine Using Systems Biology Approaches |
title_sort | comparing the primary and recall immune response induced by a new ev71 vaccine using systems biology approaches |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4605509/ https://www.ncbi.nlm.nih.gov/pubmed/26465882 http://dx.doi.org/10.1371/journal.pone.0140515 |
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