Cargando…
Localization of ORC1 During the Cell Cycle in Human Leukemia Cells
The interaction of the origin recognition complex (ORC) with replication origins is a critical parameter in eukaryotic replication initiation. In mammals the ORC remains bound except during mitosis, thus the localization of ORC complexes allows localization of origins. A monoclonal antibody that rec...
Autores principales: | , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
IOS Press
2011
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4605756/ https://www.ncbi.nlm.nih.gov/pubmed/22045277 http://dx.doi.org/10.3233/ACP-2011-0041 |
_version_ | 1782395255756161024 |
---|---|
author | Coffman, Frederick D. Reyes, Mai-Ling Brown, Monique Lambert, W. Clark Cohen, Stanley |
author_facet | Coffman, Frederick D. Reyes, Mai-Ling Brown, Monique Lambert, W. Clark Cohen, Stanley |
author_sort | Coffman, Frederick D. |
collection | PubMed |
description | The interaction of the origin recognition complex (ORC) with replication origins is a critical parameter in eukaryotic replication initiation. In mammals the ORC remains bound except during mitosis, thus the localization of ORC complexes allows localization of origins. A monoclonal antibody that recognizes human ORC1 was used to localize ORC complexes in populations of human MOLT-4 cells separated by cell cycle position using centrifugal elutriation. ORC1 staining in cells in early G1 is diffuse and primarily peripheral. As the cells traverse G1, ORC1 accumulates and becomes more localized towards the center of the nucleus, however around the G1/S boundary the staining pattern changes and ORC1 appears peripheral. By mid to late S phase ORC1 immunofluorescence is again concentrated at the nuclear center. During anaphase, ORC1 staining is localized mainly in the pericentriolar regions. These findings suggest that concerted movements of origin DNA sequences in addition to the previously documented assembly and disassembly of protein complexes are an important aspect of replication initiation loci in eukaryotes. |
format | Online Article Text |
id | pubmed-4605756 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | IOS Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-46057562015-12-13 Localization of ORC1 During the Cell Cycle in Human Leukemia Cells Coffman, Frederick D. Reyes, Mai-Ling Brown, Monique Lambert, W. Clark Cohen, Stanley Anal Cell Pathol (Amst) Other The interaction of the origin recognition complex (ORC) with replication origins is a critical parameter in eukaryotic replication initiation. In mammals the ORC remains bound except during mitosis, thus the localization of ORC complexes allows localization of origins. A monoclonal antibody that recognizes human ORC1 was used to localize ORC complexes in populations of human MOLT-4 cells separated by cell cycle position using centrifugal elutriation. ORC1 staining in cells in early G1 is diffuse and primarily peripheral. As the cells traverse G1, ORC1 accumulates and becomes more localized towards the center of the nucleus, however around the G1/S boundary the staining pattern changes and ORC1 appears peripheral. By mid to late S phase ORC1 immunofluorescence is again concentrated at the nuclear center. During anaphase, ORC1 staining is localized mainly in the pericentriolar regions. These findings suggest that concerted movements of origin DNA sequences in addition to the previously documented assembly and disassembly of protein complexes are an important aspect of replication initiation loci in eukaryotes. IOS Press 2011 2011-11-01 /pmc/articles/PMC4605756/ /pubmed/22045277 http://dx.doi.org/10.3233/ACP-2011-0041 Text en Copyright © 2011 Hindawi Publishing Corporation and the authors. |
spellingShingle | Other Coffman, Frederick D. Reyes, Mai-Ling Brown, Monique Lambert, W. Clark Cohen, Stanley Localization of ORC1 During the Cell Cycle in Human Leukemia Cells |
title | Localization of ORC1 During the Cell Cycle in Human Leukemia Cells |
title_full | Localization of ORC1 During the Cell Cycle in Human Leukemia Cells |
title_fullStr | Localization of ORC1 During the Cell Cycle in Human Leukemia Cells |
title_full_unstemmed | Localization of ORC1 During the Cell Cycle in Human Leukemia Cells |
title_short | Localization of ORC1 During the Cell Cycle in Human Leukemia Cells |
title_sort | localization of orc1 during the cell cycle in human leukemia cells |
topic | Other |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4605756/ https://www.ncbi.nlm.nih.gov/pubmed/22045277 http://dx.doi.org/10.3233/ACP-2011-0041 |
work_keys_str_mv | AT coffmanfrederickd localizationoforc1duringthecellcycleinhumanleukemiacells AT reyesmailing localizationoforc1duringthecellcycleinhumanleukemiacells AT brownmonique localizationoforc1duringthecellcycleinhumanleukemiacells AT lambertwclark localizationoforc1duringthecellcycleinhumanleukemiacells AT cohenstanley localizationoforc1duringthecellcycleinhumanleukemiacells |