Use of aminoglycoside 3′ adenyltransferase as a selection marker for Chlamydia trachomatis intron-mutagenesis and in vivo intron stability

BACKGROUND: Chlamydia spp. are obligate, intracellular bacteria that infect humans and animals. Research on these important pathogens has been hindered due to a paucity of genetic tools. We recently adapted a group II intron (GII) mutagenesis platform for creation of ampicillin-selectable gene inser...

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Detalles Bibliográficos
Autores principales: Lowden, Nicole M., Yeruva, Laxmi, Johnson, Cayla M., Bowlin, Anne K., Fisher, Derek J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2015
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4606545/
https://www.ncbi.nlm.nih.gov/pubmed/26471806
http://dx.doi.org/10.1186/s13104-015-1542-9

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4606545/
https://www.ncbi.nlm.nih.gov/pubmed/26471806
http://dx.doi.org/10.1186/s13104-015-1542-9

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