The autophagy GABARAPL1 gene is epigenetically regulated in breast cancer models
BACKGROUND: The GABARAP family members (GABARAP, GABARAPL1/GEC1 and GABARAPL2 /GATE-16) are involved in the intracellular transport of receptors and the autophagy pathway. We previously reported that GABARAPL1 expression was frequently downregulated in cancer cells while a high GABARAPL1 expression...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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BioMed Central
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4609056/ https://www.ncbi.nlm.nih.gov/pubmed/26474850 http://dx.doi.org/10.1186/s12885-015-1761-4 |
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author | Hervouet, Eric Claude-Taupin, Aurore Gauthier, Thierry Perez, Valérie Fraichard, Annick Adami, Pascale Despouy, Gilles Monnien, Franck Algros, Marie-Paule Jouvenot, Michèle Delage-Mourroux, Régis Boyer-Guittaut, Michaël |
author_facet | Hervouet, Eric Claude-Taupin, Aurore Gauthier, Thierry Perez, Valérie Fraichard, Annick Adami, Pascale Despouy, Gilles Monnien, Franck Algros, Marie-Paule Jouvenot, Michèle Delage-Mourroux, Régis Boyer-Guittaut, Michaël |
author_sort | Hervouet, Eric |
collection | PubMed |
description | BACKGROUND: The GABARAP family members (GABARAP, GABARAPL1/GEC1 and GABARAPL2 /GATE-16) are involved in the intracellular transport of receptors and the autophagy pathway. We previously reported that GABARAPL1 expression was frequently downregulated in cancer cells while a high GABARAPL1 expression is a good prognosis marker for patients with lymph node-positive breast cancer. METHODS: In this study, we asked using qRT-PCR, western blotting and epigenetic quantification whether the expression of the GABARAP family was regulated in breast cancer by epigenetic modifications. RESULTS: Our data demonstrated that a specific decrease of GABARAPL1 expression in breast cancers was associated with both DNA methylation and histone deacetylation and that CREB-1 recruitment on GABARAPL1 promoter was required for GABARAPL1 expression. CONCLUSIONS: Our work strongly suggests that epigenetic inhibitors and CREB-1 modulators may be used in the future to regulate autophagy in breast cancer cells. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12885-015-1761-4) contains supplementary material, which is available to authorized users. |
format | Online Article Text |
id | pubmed-4609056 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-46090562015-10-18 The autophagy GABARAPL1 gene is epigenetically regulated in breast cancer models Hervouet, Eric Claude-Taupin, Aurore Gauthier, Thierry Perez, Valérie Fraichard, Annick Adami, Pascale Despouy, Gilles Monnien, Franck Algros, Marie-Paule Jouvenot, Michèle Delage-Mourroux, Régis Boyer-Guittaut, Michaël BMC Cancer Research Article BACKGROUND: The GABARAP family members (GABARAP, GABARAPL1/GEC1 and GABARAPL2 /GATE-16) are involved in the intracellular transport of receptors and the autophagy pathway. We previously reported that GABARAPL1 expression was frequently downregulated in cancer cells while a high GABARAPL1 expression is a good prognosis marker for patients with lymph node-positive breast cancer. METHODS: In this study, we asked using qRT-PCR, western blotting and epigenetic quantification whether the expression of the GABARAP family was regulated in breast cancer by epigenetic modifications. RESULTS: Our data demonstrated that a specific decrease of GABARAPL1 expression in breast cancers was associated with both DNA methylation and histone deacetylation and that CREB-1 recruitment on GABARAPL1 promoter was required for GABARAPL1 expression. CONCLUSIONS: Our work strongly suggests that epigenetic inhibitors and CREB-1 modulators may be used in the future to regulate autophagy in breast cancer cells. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12885-015-1761-4) contains supplementary material, which is available to authorized users. BioMed Central 2015-10-17 /pmc/articles/PMC4609056/ /pubmed/26474850 http://dx.doi.org/10.1186/s12885-015-1761-4 Text en © Hervouet et al. 2015 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Article Hervouet, Eric Claude-Taupin, Aurore Gauthier, Thierry Perez, Valérie Fraichard, Annick Adami, Pascale Despouy, Gilles Monnien, Franck Algros, Marie-Paule Jouvenot, Michèle Delage-Mourroux, Régis Boyer-Guittaut, Michaël The autophagy GABARAPL1 gene is epigenetically regulated in breast cancer models |
title | The autophagy GABARAPL1 gene is epigenetically regulated in breast cancer models |
title_full | The autophagy GABARAPL1 gene is epigenetically regulated in breast cancer models |
title_fullStr | The autophagy GABARAPL1 gene is epigenetically regulated in breast cancer models |
title_full_unstemmed | The autophagy GABARAPL1 gene is epigenetically regulated in breast cancer models |
title_short | The autophagy GABARAPL1 gene is epigenetically regulated in breast cancer models |
title_sort | autophagy gabarapl1 gene is epigenetically regulated in breast cancer models |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4609056/ https://www.ncbi.nlm.nih.gov/pubmed/26474850 http://dx.doi.org/10.1186/s12885-015-1761-4 |
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