Cargando…

A novel IgY-Aptamer hybrid system for cost-effective detection of SEB and its evaluation on food and clinical samples

In the present study, we introduce a novel hybrid sandwich-ALISA employing chicken IgY and ssDNA aptamers for the detection of staphylococcal enterotoxin B (SEB). Cloning, expression and purification of the full length recombinant SEB was carried out. Anti-SEB IgY antibodies generated by immunizing...

Descripción completa

Detalles Bibliográficos
Autores principales: Mudili, Venkataramana, Makam, Shivakiran S., Sundararaj, Naveen, Siddaiah, Chandranayaka, Gupta, Vijai Kumar, Rao, Putcha V. Lakshmana
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4609960/
https://www.ncbi.nlm.nih.gov/pubmed/26477645
http://dx.doi.org/10.1038/srep15151