Avoidance and Potential Remedy Solutions of Chimeras in Reconstructing the Phylogeny of Aphids Using the 16S rRNA Gene of Buchnera: A Case in Lachninae (Hemiptera)

It is known that PCR amplification of highly homologous genes from complex DNA mixtures can generate a significant proportion of chimeric sequences. The 16S rRNA gene is not only widely used in estimating the species diversity of endosymbionts in aphids but also used to explore the co-diversificatio...

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Detalles Bibliográficos
Autores principales: Chen, Rui, Wang, Zhe, Chen, Jing, Qiao, Ge-Xia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2015
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4613194/
https://www.ncbi.nlm.nih.gov/pubmed/26307984
http://dx.doi.org/10.3390/ijms160920152

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4613194/
https://www.ncbi.nlm.nih.gov/pubmed/26307984
http://dx.doi.org/10.3390/ijms160920152

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