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The NHERF2 sequence adjacent and upstream of the ERM-binding domain affects NHERF2–ezrin binding and dexamethasone stimulated NHE3 activity
In the brush border of intestinal and kidney epithelial cells, scaffolding proteins ezrin, Na(+)-H(+) exchanger regulatory factor (NHERF)1 and NHERF2 play important roles in linking transmembrane proteins to the cytoskeleton and assembling signalling regulatory complexes. The last 30 carboxyl residu...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Portland Press Ltd.
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4613507/ https://www.ncbi.nlm.nih.gov/pubmed/26251448 http://dx.doi.org/10.1042/BJ20150238 |
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author | Yang, Jianbo Sarker, Rafiquel Singh, Varsha Sarker, Prateeti Yin, Jianyi Chen, Tian-E Chaerkady, Raghothama Li, Xuhang Tse, C. Ming Donowitz, Mark |
author_facet | Yang, Jianbo Sarker, Rafiquel Singh, Varsha Sarker, Prateeti Yin, Jianyi Chen, Tian-E Chaerkady, Raghothama Li, Xuhang Tse, C. Ming Donowitz, Mark |
author_sort | Yang, Jianbo |
collection | PubMed |
description | In the brush border of intestinal and kidney epithelial cells, scaffolding proteins ezrin, Na(+)-H(+) exchanger regulatory factor (NHERF)1 and NHERF2 play important roles in linking transmembrane proteins to the cytoskeleton and assembling signalling regulatory complexes. The last 30 carboxyl residues of NHERF1 and NHERF2 form the EBDs [ezrin, radixin and moesin (ERM)-binding domain]. The current study found that NHERF1/2 contain an ERM-binding regulatory sequence (EBRS), which facilitates the interaction between the EBD and ezrin. The EBRSs are located within 24 and 19 residues immediately upstream of EBDs for NHERF1 and NHERF2 respectively. In OK (opossum kidney) epithelial cells, EBRSs are necessary along with the EBD to distribute NHERF1 and NHERF2 exclusively to the apical domain. Furthermore, phosphorylation of Ser(303) located in the EBRS of NHERF2, decreases the binding affinity for ezrin, dislocates apical NHERF2 into the cytosol and increases the NHERF2 microvillar mobility rate. Moreover, increased phosphorylation of Ser(303) was functionally significant preventing acute stimulation of NHE3 (Na(+)-H(+) exchanger 3) activity by dexamethasone. |
format | Online Article Text |
id | pubmed-4613507 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Portland Press Ltd. |
record_format | MEDLINE/PubMed |
spelling | pubmed-46135072015-10-23 The NHERF2 sequence adjacent and upstream of the ERM-binding domain affects NHERF2–ezrin binding and dexamethasone stimulated NHE3 activity Yang, Jianbo Sarker, Rafiquel Singh, Varsha Sarker, Prateeti Yin, Jianyi Chen, Tian-E Chaerkady, Raghothama Li, Xuhang Tse, C. Ming Donowitz, Mark Biochem J Research Articles In the brush border of intestinal and kidney epithelial cells, scaffolding proteins ezrin, Na(+)-H(+) exchanger regulatory factor (NHERF)1 and NHERF2 play important roles in linking transmembrane proteins to the cytoskeleton and assembling signalling regulatory complexes. The last 30 carboxyl residues of NHERF1 and NHERF2 form the EBDs [ezrin, radixin and moesin (ERM)-binding domain]. The current study found that NHERF1/2 contain an ERM-binding regulatory sequence (EBRS), which facilitates the interaction between the EBD and ezrin. The EBRSs are located within 24 and 19 residues immediately upstream of EBDs for NHERF1 and NHERF2 respectively. In OK (opossum kidney) epithelial cells, EBRSs are necessary along with the EBD to distribute NHERF1 and NHERF2 exclusively to the apical domain. Furthermore, phosphorylation of Ser(303) located in the EBRS of NHERF2, decreases the binding affinity for ezrin, dislocates apical NHERF2 into the cytosol and increases the NHERF2 microvillar mobility rate. Moreover, increased phosphorylation of Ser(303) was functionally significant preventing acute stimulation of NHE3 (Na(+)-H(+) exchanger 3) activity by dexamethasone. Portland Press Ltd. 2015-08-06 2015-08-15 /pmc/articles/PMC4613507/ /pubmed/26251448 http://dx.doi.org/10.1042/BJ20150238 Text en © 2015 Authors; published by Portland Press Limited |
spellingShingle | Research Articles Yang, Jianbo Sarker, Rafiquel Singh, Varsha Sarker, Prateeti Yin, Jianyi Chen, Tian-E Chaerkady, Raghothama Li, Xuhang Tse, C. Ming Donowitz, Mark The NHERF2 sequence adjacent and upstream of the ERM-binding domain affects NHERF2–ezrin binding and dexamethasone stimulated NHE3 activity |
title | The NHERF2 sequence adjacent and upstream of the ERM-binding domain affects NHERF2–ezrin binding and dexamethasone stimulated NHE3 activity |
title_full | The NHERF2 sequence adjacent and upstream of the ERM-binding domain affects NHERF2–ezrin binding and dexamethasone stimulated NHE3 activity |
title_fullStr | The NHERF2 sequence adjacent and upstream of the ERM-binding domain affects NHERF2–ezrin binding and dexamethasone stimulated NHE3 activity |
title_full_unstemmed | The NHERF2 sequence adjacent and upstream of the ERM-binding domain affects NHERF2–ezrin binding and dexamethasone stimulated NHE3 activity |
title_short | The NHERF2 sequence adjacent and upstream of the ERM-binding domain affects NHERF2–ezrin binding and dexamethasone stimulated NHE3 activity |
title_sort | nherf2 sequence adjacent and upstream of the erm-binding domain affects nherf2–ezrin binding and dexamethasone stimulated nhe3 activity |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4613507/ https://www.ncbi.nlm.nih.gov/pubmed/26251448 http://dx.doi.org/10.1042/BJ20150238 |
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