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3D spheroid cultures improve the metabolic gene expression profiles of HepaRG cells
3D (three-dimensional) cultures are considered to be an effective method for toxicological studies; however, little evidence has been reported whether 3D cultures have an impact on hepatocellular physiology regarding lipid or glucose metabolism. In the present study, we conducted physiological chara...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Portland Press Ltd.
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4613666/ https://www.ncbi.nlm.nih.gov/pubmed/26182370 http://dx.doi.org/10.1042/BSR20150034 |
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author | Takahashi, Yu Hori, Yuji Yamamoto, Tomohisa Urashima, Toshiki Ohara, Yasunori Tanaka, Hideo |
author_facet | Takahashi, Yu Hori, Yuji Yamamoto, Tomohisa Urashima, Toshiki Ohara, Yasunori Tanaka, Hideo |
author_sort | Takahashi, Yu |
collection | PubMed |
description | 3D (three-dimensional) cultures are considered to be an effective method for toxicological studies; however, little evidence has been reported whether 3D cultures have an impact on hepatocellular physiology regarding lipid or glucose metabolism. In the present study, we conducted physiological characterization of hepatoma cell lines HepG2 and HepaRG cells cultured in 3D conditions using a hanging drop method to verify the effect of culture environment on cellular responses. Apo (Apolipoprotein)B as well as albumin secretion was augmented by 3D cultures. Expression of genes related to not only drug, but also glucose and lipid metabolism were significantly enhanced in 3D cultured HepaRG spheroids. Furthermore, mRNA levels of CYP (cytochrome P450) enzymes following exposure to corresponding inducers increased under the 3D condition. These data suggest that this simple 3D culture system without any special biomaterials can improve liver-specific characteristics including lipid metabolism. Considering that the system enables high-throughput assay, it may become a powerful tool for compound screening concerning hepatocellular responses in order to identify potential drugs. |
format | Online Article Text |
id | pubmed-4613666 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Portland Press Ltd. |
record_format | MEDLINE/PubMed |
spelling | pubmed-46136662016-09-13 3D spheroid cultures improve the metabolic gene expression profiles of HepaRG cells Takahashi, Yu Hori, Yuji Yamamoto, Tomohisa Urashima, Toshiki Ohara, Yasunori Tanaka, Hideo Biosci Rep Original Papers 3D (three-dimensional) cultures are considered to be an effective method for toxicological studies; however, little evidence has been reported whether 3D cultures have an impact on hepatocellular physiology regarding lipid or glucose metabolism. In the present study, we conducted physiological characterization of hepatoma cell lines HepG2 and HepaRG cells cultured in 3D conditions using a hanging drop method to verify the effect of culture environment on cellular responses. Apo (Apolipoprotein)B as well as albumin secretion was augmented by 3D cultures. Expression of genes related to not only drug, but also glucose and lipid metabolism were significantly enhanced in 3D cultured HepaRG spheroids. Furthermore, mRNA levels of CYP (cytochrome P450) enzymes following exposure to corresponding inducers increased under the 3D condition. These data suggest that this simple 3D culture system without any special biomaterials can improve liver-specific characteristics including lipid metabolism. Considering that the system enables high-throughput assay, it may become a powerful tool for compound screening concerning hepatocellular responses in order to identify potential drugs. Portland Press Ltd. 2015-06-11 /pmc/articles/PMC4613666/ /pubmed/26182370 http://dx.doi.org/10.1042/BSR20150034 Text en © 2015 Authors http://creativecommons.org/licenses/by/3.0/ This is an open access article published by Portland Press Limited and distributed under the Creative Commons Attribution Licence 3.0 (http://creativecommons.org/licenses/by/3.0/) . |
spellingShingle | Original Papers Takahashi, Yu Hori, Yuji Yamamoto, Tomohisa Urashima, Toshiki Ohara, Yasunori Tanaka, Hideo 3D spheroid cultures improve the metabolic gene expression profiles of HepaRG cells |
title | 3D spheroid cultures improve the metabolic gene expression profiles of HepaRG cells |
title_full | 3D spheroid cultures improve the metabolic gene expression profiles of HepaRG cells |
title_fullStr | 3D spheroid cultures improve the metabolic gene expression profiles of HepaRG cells |
title_full_unstemmed | 3D spheroid cultures improve the metabolic gene expression profiles of HepaRG cells |
title_short | 3D spheroid cultures improve the metabolic gene expression profiles of HepaRG cells |
title_sort | 3d spheroid cultures improve the metabolic gene expression profiles of heparg cells |
topic | Original Papers |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4613666/ https://www.ncbi.nlm.nih.gov/pubmed/26182370 http://dx.doi.org/10.1042/BSR20150034 |
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