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RNA Amplification Protocol Leads to Biased Polymerase Chain Reaction Results Especially for Low-Copy Transcripts of Human Bone Marrow-Derived Stromal Cells

The amplification of RNA is becoming increasingly important, as often only limited amounts of cells are available for gene expression analysis. In this study, the gene expression profile of the 39 human homeobox (HOX) genes was analyzed in bone marrow-derived multipotent stromal cells (BM-MSCs) by r...

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Detalles Bibliográficos
Autores principales: Coenen, Carolin, Liedtke, Stefanie, Kogler, Gesine
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4613830/
https://www.ncbi.nlm.nih.gov/pubmed/26485654
http://dx.doi.org/10.1371/journal.pone.0141070