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A LCMT1-PME-1 methylation equilibrium controls mitotic spindle size
Leucine carboxyl methyltransferase-1 (LCMT1) and protein phosphatase methylesterase-1 (PME-1) are essential enzymes that regulate the methylation of the protein phosphatase 2A catalytic subunit (PP2AC). LCMT1 and PME-1 have been linked to the regulation of cell growth and proliferation, but the unde...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Taylor & Francis
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4614068/ https://www.ncbi.nlm.nih.gov/pubmed/25839665 http://dx.doi.org/10.1080/15384101.2015.1026487 |
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author | Xia, Xiaoyu Gholkar, Ankur Senese, Silvia Torres, Jorge Z |
author_facet | Xia, Xiaoyu Gholkar, Ankur Senese, Silvia Torres, Jorge Z |
author_sort | Xia, Xiaoyu |
collection | PubMed |
description | Leucine carboxyl methyltransferase-1 (LCMT1) and protein phosphatase methylesterase-1 (PME-1) are essential enzymes that regulate the methylation of the protein phosphatase 2A catalytic subunit (PP2AC). LCMT1 and PME-1 have been linked to the regulation of cell growth and proliferation, but the underlying mechanisms have remained elusive. We show here an important role for an LCMT1-PME-1 methylation equilibrium in controlling mitotic spindle size. Depletion of LCMT1 or overexpression of PME-1 led to long spindles. In contrast, depletion of PME-1, pharmacological inhibition of PME-1 or overexpression of LCMT1 led to short spindles. Furthermore, perturbation of the LCMT1-PME-1 methylation equilibrium led to mitotic arrest, spindle assembly checkpoint activation, defective cell divisions, induction of apoptosis and reduced cell viability. Thus, we propose that the LCMT1-PME-1 methylation equilibrium is critical for regulating mitotic spindle size and thereby proper cell division. |
format | Online Article Text |
id | pubmed-4614068 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Taylor & Francis |
record_format | MEDLINE/PubMed |
spelling | pubmed-46140682016-02-03 A LCMT1-PME-1 methylation equilibrium controls mitotic spindle size Xia, Xiaoyu Gholkar, Ankur Senese, Silvia Torres, Jorge Z Cell Cycle Report Leucine carboxyl methyltransferase-1 (LCMT1) and protein phosphatase methylesterase-1 (PME-1) are essential enzymes that regulate the methylation of the protein phosphatase 2A catalytic subunit (PP2AC). LCMT1 and PME-1 have been linked to the regulation of cell growth and proliferation, but the underlying mechanisms have remained elusive. We show here an important role for an LCMT1-PME-1 methylation equilibrium in controlling mitotic spindle size. Depletion of LCMT1 or overexpression of PME-1 led to long spindles. In contrast, depletion of PME-1, pharmacological inhibition of PME-1 or overexpression of LCMT1 led to short spindles. Furthermore, perturbation of the LCMT1-PME-1 methylation equilibrium led to mitotic arrest, spindle assembly checkpoint activation, defective cell divisions, induction of apoptosis and reduced cell viability. Thus, we propose that the LCMT1-PME-1 methylation equilibrium is critical for regulating mitotic spindle size and thereby proper cell division. Taylor & Francis 2015-04-03 /pmc/articles/PMC4614068/ /pubmed/25839665 http://dx.doi.org/10.1080/15384101.2015.1026487 Text en © 2015 The Author(s). Published with license by Taylor & Francis Group, LLC http://creativecommons.org/licenses/by-nc/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution-Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. The moral rights of the named author(s) have been asserted. |
spellingShingle | Report Xia, Xiaoyu Gholkar, Ankur Senese, Silvia Torres, Jorge Z A LCMT1-PME-1 methylation equilibrium controls mitotic spindle size |
title | A LCMT1-PME-1 methylation equilibrium controls mitotic spindle size |
title_full | A LCMT1-PME-1 methylation equilibrium controls mitotic spindle size |
title_fullStr | A LCMT1-PME-1 methylation equilibrium controls mitotic spindle size |
title_full_unstemmed | A LCMT1-PME-1 methylation equilibrium controls mitotic spindle size |
title_short | A LCMT1-PME-1 methylation equilibrium controls mitotic spindle size |
title_sort | lcmt1-pme-1 methylation equilibrium controls mitotic spindle size |
topic | Report |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4614068/ https://www.ncbi.nlm.nih.gov/pubmed/25839665 http://dx.doi.org/10.1080/15384101.2015.1026487 |
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