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A LCMT1-PME-1 methylation equilibrium controls mitotic spindle size

Leucine carboxyl methyltransferase-1 (LCMT1) and protein phosphatase methylesterase-1 (PME-1) are essential enzymes that regulate the methylation of the protein phosphatase 2A catalytic subunit (PP2AC). LCMT1 and PME-1 have been linked to the regulation of cell growth and proliferation, but the unde...

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Autores principales: Xia, Xiaoyu, Gholkar, Ankur, Senese, Silvia, Torres, Jorge Z
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taylor & Francis 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4614068/
https://www.ncbi.nlm.nih.gov/pubmed/25839665
http://dx.doi.org/10.1080/15384101.2015.1026487
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author Xia, Xiaoyu
Gholkar, Ankur
Senese, Silvia
Torres, Jorge Z
author_facet Xia, Xiaoyu
Gholkar, Ankur
Senese, Silvia
Torres, Jorge Z
author_sort Xia, Xiaoyu
collection PubMed
description Leucine carboxyl methyltransferase-1 (LCMT1) and protein phosphatase methylesterase-1 (PME-1) are essential enzymes that regulate the methylation of the protein phosphatase 2A catalytic subunit (PP2AC). LCMT1 and PME-1 have been linked to the regulation of cell growth and proliferation, but the underlying mechanisms have remained elusive. We show here an important role for an LCMT1-PME-1 methylation equilibrium in controlling mitotic spindle size. Depletion of LCMT1 or overexpression of PME-1 led to long spindles. In contrast, depletion of PME-1, pharmacological inhibition of PME-1 or overexpression of LCMT1 led to short spindles. Furthermore, perturbation of the LCMT1-PME-1 methylation equilibrium led to mitotic arrest, spindle assembly checkpoint activation, defective cell divisions, induction of apoptosis and reduced cell viability. Thus, we propose that the LCMT1-PME-1 methylation equilibrium is critical for regulating mitotic spindle size and thereby proper cell division.
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spelling pubmed-46140682016-02-03 A LCMT1-PME-1 methylation equilibrium controls mitotic spindle size Xia, Xiaoyu Gholkar, Ankur Senese, Silvia Torres, Jorge Z Cell Cycle Report Leucine carboxyl methyltransferase-1 (LCMT1) and protein phosphatase methylesterase-1 (PME-1) are essential enzymes that regulate the methylation of the protein phosphatase 2A catalytic subunit (PP2AC). LCMT1 and PME-1 have been linked to the regulation of cell growth and proliferation, but the underlying mechanisms have remained elusive. We show here an important role for an LCMT1-PME-1 methylation equilibrium in controlling mitotic spindle size. Depletion of LCMT1 or overexpression of PME-1 led to long spindles. In contrast, depletion of PME-1, pharmacological inhibition of PME-1 or overexpression of LCMT1 led to short spindles. Furthermore, perturbation of the LCMT1-PME-1 methylation equilibrium led to mitotic arrest, spindle assembly checkpoint activation, defective cell divisions, induction of apoptosis and reduced cell viability. Thus, we propose that the LCMT1-PME-1 methylation equilibrium is critical for regulating mitotic spindle size and thereby proper cell division. Taylor & Francis 2015-04-03 /pmc/articles/PMC4614068/ /pubmed/25839665 http://dx.doi.org/10.1080/15384101.2015.1026487 Text en © 2015 The Author(s). Published with license by Taylor & Francis Group, LLC http://creativecommons.org/licenses/by-nc/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution-Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. The moral rights of the named author(s) have been asserted.
spellingShingle Report
Xia, Xiaoyu
Gholkar, Ankur
Senese, Silvia
Torres, Jorge Z
A LCMT1-PME-1 methylation equilibrium controls mitotic spindle size
title A LCMT1-PME-1 methylation equilibrium controls mitotic spindle size
title_full A LCMT1-PME-1 methylation equilibrium controls mitotic spindle size
title_fullStr A LCMT1-PME-1 methylation equilibrium controls mitotic spindle size
title_full_unstemmed A LCMT1-PME-1 methylation equilibrium controls mitotic spindle size
title_short A LCMT1-PME-1 methylation equilibrium controls mitotic spindle size
title_sort lcmt1-pme-1 methylation equilibrium controls mitotic spindle size
topic Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4614068/
https://www.ncbi.nlm.nih.gov/pubmed/25839665
http://dx.doi.org/10.1080/15384101.2015.1026487
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