Small indels induced by CRISPR/Cas9 in the 5′ region of microRNA lead to its depletion and Drosha processing retardance

MicroRNA knockout by genome editing technologies is promising. In order to extend the application of the technology and to investigate the function of a specific miRNA, we used CRISPR/Cas9 to deplete human miR-93 from a cluster by targeting its 5’ region in HeLa cells. Various small indels were indu...

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Detalles Bibliográficos
Autores principales: Jiang, Qian, Meng, Xing, Meng, Lingwei, Chang, Nannan, Xiong, Jingwei, Cao, Huiqing, Liang, Zicai
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taylor & Francis 2015
Materias:
Brief Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4615719/
https://www.ncbi.nlm.nih.gov/pubmed/25590615
http://dx.doi.org/10.1080/15476286.2014.996067

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4615719/
https://www.ncbi.nlm.nih.gov/pubmed/25590615
http://dx.doi.org/10.1080/15476286.2014.996067

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