Optimized production of transgenic buffalo embryos and offspring by cytoplasmic zygote injection

BACKGROUND: Cytoplasmic injection of exogenous DNA into zygotes is a promising technique to generate transgenic livestock. However, it is still relatively inefficient and has not yet been demonstrated to work in buffalo. We sought to improve two key technical parameters of the procedure, namely i) h...

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Detalles Bibliográficos
Autores principales: Meng, Fanli, Li, Hui, Wang, Xiaoli, Qin, Guangsheng, Oback, Björn, Shi, Deshun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2015
Materias:
Methodology Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4617447/
https://www.ncbi.nlm.nih.gov/pubmed/26500768
http://dx.doi.org/10.1186/s40104-015-0044-x

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4617447/
https://www.ncbi.nlm.nih.gov/pubmed/26500768
http://dx.doi.org/10.1186/s40104-015-0044-x

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