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Optimized production of transgenic buffalo embryos and offspring by cytoplasmic zygote injection
BACKGROUND: Cytoplasmic injection of exogenous DNA into zygotes is a promising technique to generate transgenic livestock. However, it is still relatively inefficient and has not yet been demonstrated to work in buffalo. We sought to improve two key technical parameters of the procedure, namely i) h...
Autores principales: | Meng, Fanli, Li, Hui, Wang, Xiaoli, Qin, Guangsheng, Oback, Björn, Shi, Deshun |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4617447/ https://www.ncbi.nlm.nih.gov/pubmed/26500768 http://dx.doi.org/10.1186/s40104-015-0044-x |
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