High Resolution Comparative Genomic Hybridization Detects 7–8 Megabasepair Deletion in PCR Amplified DNA(1)

We investigated if any change in spatial resolution of comparative genomic hybridization analysis could be detected when using DNA amplified by degenerate oligonucleotide primed PCR (DOP‐PCR) as opposed to the use of unamplified DNA. Five DNA samples from B‐cell leukemias with small 11q deletions we...

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Detalles Bibliográficos
Autores principales: Larsen, Jacob, Ottesen, Anne Marie, Kirchhoff, Maria, Lundsteen, Claes, Larsen, Jørgen K.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: IOS Press 2001
Materias:
Other
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4618809/
https://www.ncbi.nlm.nih.gov/pubmed/11904461
http://dx.doi.org/10.1155/2001/301570
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author Larsen, Jacob
Ottesen, Anne Marie
Kirchhoff, Maria
Lundsteen, Claes
Larsen, Jørgen K.
author_facet Larsen, Jacob
Ottesen, Anne Marie
Kirchhoff, Maria
Lundsteen, Claes
Larsen, Jørgen K.
author_sort Larsen, Jacob
collection PubMed
description We investigated if any change in spatial resolution of comparative genomic hybridization analysis could be detected when using DNA amplified by degenerate oligonucleotide primed PCR (DOP‐PCR) as opposed to the use of unamplified DNA. Five DNA samples from B‐cell leukemias with small 11q deletions were amplified by DOP‐PCR and analysed by means of high resolution comparative genomic hybridization (HR‐CGH) for the evaluation of aberration size detection limit. By means of HR‐CGH, we found the detection limit of DOP‐PCR CGH for deletions to be between 3 Mbp and 7–8 Mbp.
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spelling pubmed-46188092016-01-12 High Resolution Comparative Genomic Hybridization Detects 7–8 Megabasepair Deletion in PCR Amplified DNA(1) Larsen, Jacob Ottesen, Anne Marie Kirchhoff, Maria Lundsteen, Claes Larsen, Jørgen K. Anal Cell Pathol Other We investigated if any change in spatial resolution of comparative genomic hybridization analysis could be detected when using DNA amplified by degenerate oligonucleotide primed PCR (DOP‐PCR) as opposed to the use of unamplified DNA. Five DNA samples from B‐cell leukemias with small 11q deletions were amplified by DOP‐PCR and analysed by means of high resolution comparative genomic hybridization (HR‐CGH) for the evaluation of aberration size detection limit. By means of HR‐CGH, we found the detection limit of DOP‐PCR CGH for deletions to be between 3 Mbp and 7–8 Mbp. IOS Press 2001 2001-01-01 /pmc/articles/PMC4618809/ /pubmed/11904461 http://dx.doi.org/10.1155/2001/301570 Text en Copyright © 2001 Hindawi Publishing Corporation.
spellingShingle Other
Larsen, Jacob
Ottesen, Anne Marie
Kirchhoff, Maria
Lundsteen, Claes
Larsen, Jørgen K.
High Resolution Comparative Genomic Hybridization Detects 7–8 Megabasepair Deletion in PCR Amplified DNA(1)
title High Resolution Comparative Genomic Hybridization Detects 7–8 Megabasepair Deletion in PCR Amplified DNA(1)
title_full High Resolution Comparative Genomic Hybridization Detects 7–8 Megabasepair Deletion in PCR Amplified DNA(1)
title_fullStr High Resolution Comparative Genomic Hybridization Detects 7–8 Megabasepair Deletion in PCR Amplified DNA(1)
title_full_unstemmed High Resolution Comparative Genomic Hybridization Detects 7–8 Megabasepair Deletion in PCR Amplified DNA(1)
title_short High Resolution Comparative Genomic Hybridization Detects 7–8 Megabasepair Deletion in PCR Amplified DNA(1)
title_sort high resolution comparative genomic hybridization detects 7–8 megabasepair deletion in pcr amplified dna(1)
topic Other
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4618809/
https://www.ncbi.nlm.nih.gov/pubmed/11904461
http://dx.doi.org/10.1155/2001/301570
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