Detection of Alicyclobacillus spp. in Fruit Juice by Combination of Immunomagnetic Separation and a SYBR Green I Real-Time PCR Assay

An approach based on immunomagnetic separation (IMS) and SYBR Green I real-time PCR (real-time PCR) with species-specific primers and melting curve analysis was proposed as a rapid and effective method for detecting Alicyclobacillus spp. in fruit juices. Specific primers targeting the 16S rDNA seque...

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Detalles Bibliográficos
Autores principales: Cai, Rui, Wang, Zhouli, Yuan, Yahong, Liu, Bin, Wang, Ling, Yue, Tianli
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2015
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4619346/
https://www.ncbi.nlm.nih.gov/pubmed/26488469
http://dx.doi.org/10.1371/journal.pone.0141049

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4619346/
https://www.ncbi.nlm.nih.gov/pubmed/26488469
http://dx.doi.org/10.1371/journal.pone.0141049

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