Degradation of C-terminal tag sequences on domain antibodies purified from E. coli supernatant

Expression of recombinant proteins often takes advantage of peptide tags expressed in fusion to allow easy detection and purification of the expressed proteins. However, as the fusion peptides most often are flexible appendages at the N- or C-terminal, proteolytic cleavage may result in removal of t...

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Detalles Bibliográficos
Autores principales: Lykkemark, Simon, Mandrup, Ole Aalund, Friis, Niels Anton, Kristensen, Peter
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taylor & Francis 2014
Materias:
Reports
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4622476/
https://www.ncbi.nlm.nih.gov/pubmed/25426869
http://dx.doi.org/10.4161/mabs.36211

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4622476/
https://www.ncbi.nlm.nih.gov/pubmed/25426869
http://dx.doi.org/10.4161/mabs.36211

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