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Degradation of C-terminal tag sequences on domain antibodies purified from E. coli supernatant
Expression of recombinant proteins often takes advantage of peptide tags expressed in fusion to allow easy detection and purification of the expressed proteins. However, as the fusion peptides most often are flexible appendages at the N- or C-terminal, proteolytic cleavage may result in removal of t...
| Autores principales: | , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Taylor & Francis
2014
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4622476/ https://www.ncbi.nlm.nih.gov/pubmed/25426869 http://dx.doi.org/10.4161/mabs.36211 |