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A generic approach to engineer antibody pH-switches using combinatorial histidine scanning libraries and yeast display
There is growing interest in the fast and robust engineering of protein pH-sensitivity that aims to reduce binding at acidic pH, compared to neutral pH. Here, we describe a novel strategy for the incorporation of pH-sensitive antigen binding functions into antibody variable domains using combinatori...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Taylor & Francis
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4622719/ https://www.ncbi.nlm.nih.gov/pubmed/25523975 http://dx.doi.org/10.4161/19420862.2014.985993 |