A generic approach to engineer antibody pH-switches using combinatorial histidine scanning libraries and yeast display

There is growing interest in the fast and robust engineering of protein pH-sensitivity that aims to reduce binding at acidic pH, compared to neutral pH. Here, we describe a novel strategy for the incorporation of pH-sensitive antigen binding functions into antibody variable domains using combinatori...

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Detalles Bibliográficos
Autores principales: Schröter, Christian, Günther, Ralf, Rhiel, Laura, Becker, Stefan, Toleikis, Lars, Doerner, Achim, Becker, Janine, Schönemann, Andreas, Nasu, Daichi, Neuteboom, Berend, Kolmar, Harald, Hock, Björn
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taylor & Francis 2014
Materias:
Reports
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4622719/
https://www.ncbi.nlm.nih.gov/pubmed/25523975
http://dx.doi.org/10.4161/19420862.2014.985993

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