Functionalized micro-capillary film for the rapid at-line analysis of IgG aggregates in a cell culture bioreactor

A micro-capillary film has been developed that offers the potential for an at-line analytical tool for rapid aggregate analysis during biopharmaceutical antibody production. A non-porous walled micro-capillary film (NMCF) with cation exchange functionality was demonstrated to act as a chromatography...

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Autores principales: Townsend, Matthew J, Gruber, David E, Kuiper, Marcel, Lazar, Radu A, Field, Ray P, Turner, Richard E, Slater, Nigel KH
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taylor & Francis 2015
Materias:
Short Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4623336/
https://www.ncbi.nlm.nih.gov/pubmed/26176737
http://dx.doi.org/10.1080/19420862.2015.1065365
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author Townsend, Matthew J
Gruber, David E
Kuiper, Marcel
Lazar, Radu A
Field, Ray P
Turner, Richard E
Slater, Nigel KH
author_facet Townsend, Matthew J
Gruber, David E
Kuiper, Marcel
Lazar, Radu A
Field, Ray P
Turner, Richard E
Slater, Nigel KH
author_sort Townsend, Matthew J
collection PubMed
description A micro-capillary film has been developed that offers the potential for an at-line analytical tool for rapid aggregate analysis during biopharmaceutical antibody production. A non-porous walled micro-capillary film (NMCF) with cation exchange functionality was demonstrated to act as a chromatography medium that could be operated with high linear fluid velocities and was highly resistant to blockage by entrained particulates, including cells. The NMCF containing 19 parallel microcapillaries was prepared using a melt extrusion process from poly(ethylene-vinyl alcohol) copolymer (EVOH). The NMCF-EVOH was modified to have cation-exchange functionality (NMCF-EVOH-SP) and shown to differentially bind monomer and aggregated species of IgG antibody directly from a bioreactor. The use of NMCF-EVOH-SP to quantify aggregate concentrations in monoclonal antibody preparations in less than 20 minutes was demonstrated.
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spelling pubmed-46233362016-02-03 Functionalized micro-capillary film for the rapid at-line analysis of IgG aggregates in a cell culture bioreactor Townsend, Matthew J Gruber, David E Kuiper, Marcel Lazar, Radu A Field, Ray P Turner, Richard E Slater, Nigel KH MAbs Short Communication A micro-capillary film has been developed that offers the potential for an at-line analytical tool for rapid aggregate analysis during biopharmaceutical antibody production. A non-porous walled micro-capillary film (NMCF) with cation exchange functionality was demonstrated to act as a chromatography medium that could be operated with high linear fluid velocities and was highly resistant to blockage by entrained particulates, including cells. The NMCF containing 19 parallel microcapillaries was prepared using a melt extrusion process from poly(ethylene-vinyl alcohol) copolymer (EVOH). The NMCF-EVOH was modified to have cation-exchange functionality (NMCF-EVOH-SP) and shown to differentially bind monomer and aggregated species of IgG antibody directly from a bioreactor. The use of NMCF-EVOH-SP to quantify aggregate concentrations in monoclonal antibody preparations in less than 20 minutes was demonstrated. Taylor & Francis 2015-07-15 /pmc/articles/PMC4623336/ /pubmed/26176737 http://dx.doi.org/10.1080/19420862.2015.1065365 Text en © 2015 The Author(s). Published with license by Taylor & Francis Group, LLC http://creativecommons.org/licenses/by/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The moral rights of the named author(s) have been asserted.
spellingShingle Short Communication
Townsend, Matthew J
Gruber, David E
Kuiper, Marcel
Lazar, Radu A
Field, Ray P
Turner, Richard E
Slater, Nigel KH
Functionalized micro-capillary film for the rapid at-line analysis of IgG aggregates in a cell culture bioreactor
title Functionalized micro-capillary film for the rapid at-line analysis of IgG aggregates in a cell culture bioreactor
title_full Functionalized micro-capillary film for the rapid at-line analysis of IgG aggregates in a cell culture bioreactor
title_fullStr Functionalized micro-capillary film for the rapid at-line analysis of IgG aggregates in a cell culture bioreactor
title_full_unstemmed Functionalized micro-capillary film for the rapid at-line analysis of IgG aggregates in a cell culture bioreactor
title_short Functionalized micro-capillary film for the rapid at-line analysis of IgG aggregates in a cell culture bioreactor
title_sort functionalized micro-capillary film for the rapid at-line analysis of igg aggregates in a cell culture bioreactor
topic Short Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4623336/
https://www.ncbi.nlm.nih.gov/pubmed/26176737
http://dx.doi.org/10.1080/19420862.2015.1065365
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