Cloning, expression and characterization of l-asparaginase from Pseudomonas fluorescens for large scale production in E. coli BL21

l-Asparaginase (E.C. 3.5.1.1) is used as an anti-neoplastic drug in the treatment of acute lymphoblastic leukemia. l-Asparaginase from Pseudomonas fluorescens was cloned and overexpressed in E. coli BL21. The Enzyme was found to be a Fusion protein-asparaginase complex which was given a lysozyme tre...

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Detalles Bibliográficos
Autores principales: Kishore, Vijay, Nishita, K. P., Manonmani, H. K.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2015
Materias:
Original Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4624130/
https://www.ncbi.nlm.nih.gov/pubmed/28324403
http://dx.doi.org/10.1007/s13205-015-0300-y

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4624130/
https://www.ncbi.nlm.nih.gov/pubmed/28324403
http://dx.doi.org/10.1007/s13205-015-0300-y

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