Cargando…

Nuclear domain ‘knock-in’ screen for the evaluation and identification of small molecule enhancers of CRISPR-based genome editing

CRISPR is a genome-editing platform that makes use of the bacterially-derived endonuclease Cas9 to introduce DNA double-strand breaks at precise locations in the genome using complementary guide RNAs. We developed a nuclear domain knock-in screen, whereby the insertion of a gene encoding the green f...

Descripción completa

Detalles Bibliográficos
Autores principales:	Pinder, Jordan, Salsman, Jayme, Dellaire, Graham
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Oxford University Press 2015
Materias:	Molecular Biology
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4627099/ https://www.ncbi.nlm.nih.gov/pubmed/26429972 http://dx.doi.org/10.1093/nar/gkv993

Ejemplares similares

Running ‘LAPS’ Around nLD: Nuclear Lipid Droplet Form and Function
por: McPhee, Michael J., et al.
Publicado: (2022)

Lipid-associated PML structures assemble nuclear lipid droplets containing CCTα and Lipin1
por: Lee, Jonghwa, et al.
Publicado: (2020)

Inhibition of neddylation induces mitotic defects and alters MKLP1 accumulation at the midbody during cytokinesis
por: Chung, Dudley, et al.
Publicado: (2019)

Myogenic differentiation triggers PML nuclear body loss and DAXX relocalization to chromocentres
por: Salsman, Jayme, et al.
Publicado: (2017)

Selecting for CRISPR-Edited Knock-In Cells
por: Reuven, Nina, et al.
Publicado: (2022)

Genome editing in mammalian cells using the CRISPR type I-D nuclease
por: Osakabe, Keishi, et al.
Publicado: (2021)

Cancer-causing mutations in the tumor suppressor PALB2 reveal a novel cancer mechanism using a hidden nuclear export signal in the WD40 repeat motif
por: Pauty, Joris, et al.
Publicado: (2017)

Shigella Infection Interferes with SUMOylation and Increases PML-NB Number
por: Sidik, Saima M., et al.
Publicado: (2015)

A high-throughput small molecule screen identifies farrerol as a potentiator of CRISPR/Cas9-mediated genome editing
por: Zhang, Weina, et al.
Publicado: (2020)

Plant mitochondrial RNA editing factors can perform targeted C-to-U editing of nuclear transcripts in human cells
por: Lesch, Elena, et al.
Publicado: (2022)

Allele-specific genome editing using CRISPR–Cas9 is associated with loss of heterozygosity in diploid yeast
por: Gorter de Vries, Arthur R, et al.
Publicado: (2019)

Small-molecule inhibitors of histone deacetylase improve CRISPR-based adenine base editing
por: Shin, Ha Rim, et al.
Publicado: (2021)

A CRISPR Cas9 high-throughput genome editing toolkit for kinetoplastids
por: Beneke, Tom, et al.
Publicado: (2017)

Rational guide RNA engineering for small-molecule control of CRISPR/Cas9 and gene editing
por: Liu, Xingyu, et al.
Publicado: (2022)

Highly efficient genome editing via CRISPR–Cas9 in human pluripotent stem cells is achieved by transient BCL-XL overexpression
por: Li, Xiao-Lan, et al.
Publicado: (2018)

Development of a CRISPR/Cas9 System for Methylococcus capsulatus In Vivo Gene Editing
por: Tapscott, Timothy, et al.
Publicado: (2019)

Genome Editing of Veterinary Relevant Mycoplasmas Using a CRISPR-Cas Base Editor System
por: Ipoutcha, Thomas, et al.
Publicado: (2022)

Efficient generation of Knock-in/Knock-out marmoset embryo via CRISPR/Cas9 gene editing
por: Kumita, Wakako, et al.
Publicado: (2019)

Torsional sensing of small-molecule binding using magnetic tweezers
por: Lipfert, Jan, et al.
Publicado: (2010)

Genome-Wide Screen of Three Herpesviruses for Protein Subcellular Localization and Alteration of PML Nuclear Bodies
por: Salsman, Jayme, et al.
Publicado: (2008)

Mobile element warfare via CRISPR and anti-CRISPR in Pseudomonas aeruginosa
por: León, Lina M, et al.
Publicado: (2021)

Phage-mediated Delivery of Targeted sRNA Constructs to Knock Down Gene Expression in E. coli
por: Bernheim, Aude G., et al.
Publicado: (2016)

Type III-A CRISPR-associated protein Csm6 degrades cyclic hexa-adenylate activator using both CARF and HEPN domains
por: Smalakyte, Dalia, et al.
Publicado: (2020)

Nuclear protein LEDGF/p75 recognizes supercoiled DNA by a novel DNA-binding domain
por: Tsutsui, Kimiko M., et al.
Publicado: (2011)

Research Note: Development of a chicken experimental model platform for induced pluripotent stem cells by using CRISPR/Cas9-mediated NANOG knock-in reporter DF1 cells
por: Lee, Bo Ram, et al.
Publicado: (2022)

Dataset on nicotine-free, nontransgenic tobacco (Nicotiana tabacuml.) edited by CRISPR-Cas9
por: Schachtsiek, Julia, et al.
Publicado: (2019)

Anti-CRISPR AcrIF9 functions by inducing the CRISPR–Cas complex to bind DNA non-specifically
por: Lu, Wang-Ting, et al.
Publicado: (2021)

Induced topological changes in DNA complexes: influence of DNA sequences and small molecule structures
por: Hunt, Rebecca A., et al.
Publicado: (2011)

The small molecule Retro-1 enhances the pharmacological actions of antisense and splice switching oligonucleotides
por: Ming, Xin, et al.
Publicado: (2013)

Quantifying the impact of small molecule ligands on G-quadruplex stability against Bloom helicase
por: Maleki, Parastoo, et al.
Publicado: (2019)

Production of a Cloned Offspring and CRISPR/Cas9 Genome Editing of Embryonic Fibroblasts in Cattle
por: Singina, G. N., et al.
Publicado: (2021)

A novel mechanism for regulating the activity of proliferating cell nuclear antigen by a small protein
por: Li, Zhuo, et al.
Publicado: (2014)

Substrate Generation for Endonucleases of CRISPR/Cas Systems
por: Zoephel, Judith, et al.
Publicado: (2012)

CRISPR-mediated control of the bacterial initiation of replication
por: Wiktor, Jakub, et al.
Publicado: (2016)

Primed CRISPR DNA uptake in Pyrococcus furiosus
por: Garrett, Sandra, et al.
Publicado: (2020)

DNA microstructure influences selective binding of small molecules designed to target mixed-site DNA sequences
por: Laughlin-Toth, Sarah, et al.
Publicado: (2017)

Combining different CRISPR nucleases for simultaneous knock-in and base editing prevents translocations in multiplex-edited CAR T cells
por: Glaser, Viktor, et al.
Publicado: (2023)

CRISPR DNA elements controlling site-specific spacer integration and proper repeat length by a Type II CRISPR–Cas system
por: Kim, Jenny G, et al.
Publicado: (2019)

Intron RNA editing is essential for splicing in plant mitochondria
por: Castandet, Benoît, et al.
Publicado: (2010)

CRISPR interference and priming varies with individual spacer sequences
por: Xue, Chaoyou, et al.
Publicado: (2015)

Cannot write session to /tmp/vufind_sessions/sess_hudkhor9ffjddhtsjgbou4am3s