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AmpliconDuo: A Split-Sample Filtering Protocol for High-Throughput Amplicon Sequencing of Microbial Communities

High throughput sequencing (HTSeq) of small ribosomal subunit amplicons has the potential for a comprehensive characterization of microbial community compositions, down to rare species. However, the error-prone nature of the multi-step experimental process requires that the resulting raw sequences a...

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Detalles Bibliográficos
Autores principales: Lange, Anja, Jost, Steffen, Heider, Dominik, Bock, Christina, Budeus, Bettina, Schilling, Elmar, Strittmatter, Axel, Boenigk, Jens, Hoffmann, Daniel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4629888/
https://www.ncbi.nlm.nih.gov/pubmed/26523925
http://dx.doi.org/10.1371/journal.pone.0141590