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Influence of DNA methylation on positioning and DNA flexibility of nucleosomes with pericentric satellite DNA
DNA methylation occurs on CpG sites and is important to form pericentric heterochromatin domains. The satellite 2 sequence, containing seven CpG sites, is located in the pericentric region of human chromosome 1 and is highly methylated in normal cells. In contrast, the satellite 2 region is reported...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Royal Society
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4632512/ https://www.ncbi.nlm.nih.gov/pubmed/26446621 http://dx.doi.org/10.1098/rsob.150128 |
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author | Osakabe, Akihisa Adachi, Fumiya Arimura, Yasuhiro Maehara, Kazumitsu Ohkawa, Yasuyuki Kurumizaka, Hitoshi |
author_facet | Osakabe, Akihisa Adachi, Fumiya Arimura, Yasuhiro Maehara, Kazumitsu Ohkawa, Yasuyuki Kurumizaka, Hitoshi |
author_sort | Osakabe, Akihisa |
collection | PubMed |
description | DNA methylation occurs on CpG sites and is important to form pericentric heterochromatin domains. The satellite 2 sequence, containing seven CpG sites, is located in the pericentric region of human chromosome 1 and is highly methylated in normal cells. In contrast, the satellite 2 region is reportedly hypomethylated in cancer cells, suggesting that the methylation status may affect the chromatin structure around the pericentric regions in tumours. In this study, we mapped the nucleosome positioning on the satellite 2 sequence in vitro and found that DNA methylation modestly affects the distribution of the nucleosome positioning. The micrococcal nuclease assay revealed that the DNA end flexibility of the nucleosomes changes, depending on the DNA methylation status. However, the structures and thermal stabilities of the nucleosomes are unaffected by DNA methylation. These findings provide new information to understand how DNA methylation functions in regulating pericentric heterochromatin formation and maintenance in normal and malignant cells. |
format | Online Article Text |
id | pubmed-4632512 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | The Royal Society |
record_format | MEDLINE/PubMed |
spelling | pubmed-46325122015-11-24 Influence of DNA methylation on positioning and DNA flexibility of nucleosomes with pericentric satellite DNA Osakabe, Akihisa Adachi, Fumiya Arimura, Yasuhiro Maehara, Kazumitsu Ohkawa, Yasuyuki Kurumizaka, Hitoshi Open Biol Research DNA methylation occurs on CpG sites and is important to form pericentric heterochromatin domains. The satellite 2 sequence, containing seven CpG sites, is located in the pericentric region of human chromosome 1 and is highly methylated in normal cells. In contrast, the satellite 2 region is reportedly hypomethylated in cancer cells, suggesting that the methylation status may affect the chromatin structure around the pericentric regions in tumours. In this study, we mapped the nucleosome positioning on the satellite 2 sequence in vitro and found that DNA methylation modestly affects the distribution of the nucleosome positioning. The micrococcal nuclease assay revealed that the DNA end flexibility of the nucleosomes changes, depending on the DNA methylation status. However, the structures and thermal stabilities of the nucleosomes are unaffected by DNA methylation. These findings provide new information to understand how DNA methylation functions in regulating pericentric heterochromatin formation and maintenance in normal and malignant cells. The Royal Society 2015-10-07 /pmc/articles/PMC4632512/ /pubmed/26446621 http://dx.doi.org/10.1098/rsob.150128 Text en © 2015 The Authors. http://creativecommons.org/licenses/by/4.0/ Published by the Royal Society under the terms of the Creative Commons Attribution License http://creativecommons.org/licenses/by/4.0/, which permits unrestricted use, provided the original author and source are credited. |
spellingShingle | Research Osakabe, Akihisa Adachi, Fumiya Arimura, Yasuhiro Maehara, Kazumitsu Ohkawa, Yasuyuki Kurumizaka, Hitoshi Influence of DNA methylation on positioning and DNA flexibility of nucleosomes with pericentric satellite DNA |
title | Influence of DNA methylation on positioning and DNA flexibility of nucleosomes with pericentric satellite DNA |
title_full | Influence of DNA methylation on positioning and DNA flexibility of nucleosomes with pericentric satellite DNA |
title_fullStr | Influence of DNA methylation on positioning and DNA flexibility of nucleosomes with pericentric satellite DNA |
title_full_unstemmed | Influence of DNA methylation on positioning and DNA flexibility of nucleosomes with pericentric satellite DNA |
title_short | Influence of DNA methylation on positioning and DNA flexibility of nucleosomes with pericentric satellite DNA |
title_sort | influence of dna methylation on positioning and dna flexibility of nucleosomes with pericentric satellite dna |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4632512/ https://www.ncbi.nlm.nih.gov/pubmed/26446621 http://dx.doi.org/10.1098/rsob.150128 |
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