Homologous Recombination-Independent Large Gene Cassette Knock-in in CHO Cells Using TALEN and MMEJ-Directed Donor Plasmids

Gene knock-in techniques have rapidly evolved in recent years, along with the development and maturation of genome editing technology using programmable nucleases. We recently reported a novel strategy for microhomology-mediated end-joining-dependent integration of donor DNA by using TALEN or CRISPR...

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Detalles Bibliográficos
Autores principales: Sakuma, Tetsushi, Takenaga, Mitsumasa, Kawabe, Yoshinori, Nakamura, Takahiro, Kamihira, Masamichi, Yamamoto, Takashi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2015
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4632728/
https://www.ncbi.nlm.nih.gov/pubmed/26473830
http://dx.doi.org/10.3390/ijms161023849

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4632728/
https://www.ncbi.nlm.nih.gov/pubmed/26473830
http://dx.doi.org/10.3390/ijms161023849

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