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Effects of Pulsed Electric Fields (PEF) on Vitamin C and Its Antioxidant Properties
In this study, pulsed electric fields (PEF) treatments and their effects on the structure of vitamin C (VIT-C) were estimated by fluorescence and Fourier transform infrared (FT-IR) spectroscopy, the relative content of VIT-C was measured by HPLC and the antioxidant properties of treated VIT-C by DPP...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4632744/ https://www.ncbi.nlm.nih.gov/pubmed/26473846 http://dx.doi.org/10.3390/ijms161024159 |
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author | Zhang, Zhi-Hong Zeng, Xin-An Brennan, Charles S. Brennan, Margaret Han, Zhong Xiong, Xia-Yu |
author_facet | Zhang, Zhi-Hong Zeng, Xin-An Brennan, Charles S. Brennan, Margaret Han, Zhong Xiong, Xia-Yu |
author_sort | Zhang, Zhi-Hong |
collection | PubMed |
description | In this study, pulsed electric fields (PEF) treatments and their effects on the structure of vitamin C (VIT-C) were estimated by fluorescence and Fourier transform infrared (FT-IR) spectroscopy, the relative content of VIT-C was measured by HPLC and the antioxidant properties of treated VIT-C by DPPH radical scavenging as well as reducing power tests. The fluorescence intensity of treated VIT-C increased slightly compared to the untreated VIT-C. Moreover, the effect of PEF on the structure of VIT-C was observed using the FT-IR spectra. These phenomena indicated that the PEF affected the conformation of VIT-C, which promoted the VIT-C isomer transformed enol-form into keto-form. In addition, the PEF treatments did not suffer the damage to VIT-C and could slow down the oxidation process in involving of experimental conditions by HPLC. The antioxidant properties of the treated VIT-C were enhanced, which was proved by radical scavenging and also the reducing power tests. |
format | Online Article Text |
id | pubmed-4632744 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-46327442015-11-23 Effects of Pulsed Electric Fields (PEF) on Vitamin C and Its Antioxidant Properties Zhang, Zhi-Hong Zeng, Xin-An Brennan, Charles S. Brennan, Margaret Han, Zhong Xiong, Xia-Yu Int J Mol Sci Article In this study, pulsed electric fields (PEF) treatments and their effects on the structure of vitamin C (VIT-C) were estimated by fluorescence and Fourier transform infrared (FT-IR) spectroscopy, the relative content of VIT-C was measured by HPLC and the antioxidant properties of treated VIT-C by DPPH radical scavenging as well as reducing power tests. The fluorescence intensity of treated VIT-C increased slightly compared to the untreated VIT-C. Moreover, the effect of PEF on the structure of VIT-C was observed using the FT-IR spectra. These phenomena indicated that the PEF affected the conformation of VIT-C, which promoted the VIT-C isomer transformed enol-form into keto-form. In addition, the PEF treatments did not suffer the damage to VIT-C and could slow down the oxidation process in involving of experimental conditions by HPLC. The antioxidant properties of the treated VIT-C were enhanced, which was proved by radical scavenging and also the reducing power tests. MDPI 2015-10-13 /pmc/articles/PMC4632744/ /pubmed/26473846 http://dx.doi.org/10.3390/ijms161024159 Text en © 2015 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Zhang, Zhi-Hong Zeng, Xin-An Brennan, Charles S. Brennan, Margaret Han, Zhong Xiong, Xia-Yu Effects of Pulsed Electric Fields (PEF) on Vitamin C and Its Antioxidant Properties |
title | Effects of Pulsed Electric Fields (PEF) on Vitamin C and Its Antioxidant Properties |
title_full | Effects of Pulsed Electric Fields (PEF) on Vitamin C and Its Antioxidant Properties |
title_fullStr | Effects of Pulsed Electric Fields (PEF) on Vitamin C and Its Antioxidant Properties |
title_full_unstemmed | Effects of Pulsed Electric Fields (PEF) on Vitamin C and Its Antioxidant Properties |
title_short | Effects of Pulsed Electric Fields (PEF) on Vitamin C and Its Antioxidant Properties |
title_sort | effects of pulsed electric fields (pef) on vitamin c and its antioxidant properties |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4632744/ https://www.ncbi.nlm.nih.gov/pubmed/26473846 http://dx.doi.org/10.3390/ijms161024159 |
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