The physiological concentration of ferrous iron (II) alters the inhibitory effect of hydrogen peroxide on CD45, LAR and PTP1B phosphatases

Hydrogen peroxide is an important regulator of protein tyrosine phosphatase activity via reversible oxidation. However, the role of iron in this reaction has not been yet elucidated. Here we compare the influence of hydrogen peroxide and the ferrous iron (reagent for Fenton reaction) on the enzymati...

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Autores principales: Kuban-Jankowska, Alicja, Gorska, Magdalena, Jaremko, Lukasz, Jaremko, Mariusz, Tuszynski, Jack A., Wozniak, Michal
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Netherlands 2015
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Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4635172/
https://www.ncbi.nlm.nih.gov/pubmed/26407665
http://dx.doi.org/10.1007/s10534-015-9882-4
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author Kuban-Jankowska, Alicja
Gorska, Magdalena
Jaremko, Lukasz
Jaremko, Mariusz
Tuszynski, Jack A.
Wozniak, Michal
author_facet Kuban-Jankowska, Alicja
Gorska, Magdalena
Jaremko, Lukasz
Jaremko, Mariusz
Tuszynski, Jack A.
Wozniak, Michal
author_sort Kuban-Jankowska, Alicja
collection PubMed
description Hydrogen peroxide is an important regulator of protein tyrosine phosphatase activity via reversible oxidation. However, the role of iron in this reaction has not been yet elucidated. Here we compare the influence of hydrogen peroxide and the ferrous iron (reagent for Fenton reaction) on the enzymatic activity of recombinant CD45, LAR, PTP1B phosphatases and cellular CD45 in Jurkat cells. The obtained results show that ferrous iron (II) is potent inhibitor of CD45, LAR and PTP1B, but the inhibitory effect is concentration dependent. We found that the higher concentrations of ferrous iron (II) increase the inactivation of CD45, LAR and PTP1B phosphatase caused by hydrogen peroxide, but the addition of the physiological concentration (500 nM) of ferrous iron (II) has even a slightly preventive effect on the phosphatase activity against hydrogen peroxide.
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spelling pubmed-46351722015-11-10 The physiological concentration of ferrous iron (II) alters the inhibitory effect of hydrogen peroxide on CD45, LAR and PTP1B phosphatases Kuban-Jankowska, Alicja Gorska, Magdalena Jaremko, Lukasz Jaremko, Mariusz Tuszynski, Jack A. Wozniak, Michal Biometals Article Hydrogen peroxide is an important regulator of protein tyrosine phosphatase activity via reversible oxidation. However, the role of iron in this reaction has not been yet elucidated. Here we compare the influence of hydrogen peroxide and the ferrous iron (reagent for Fenton reaction) on the enzymatic activity of recombinant CD45, LAR, PTP1B phosphatases and cellular CD45 in Jurkat cells. The obtained results show that ferrous iron (II) is potent inhibitor of CD45, LAR and PTP1B, but the inhibitory effect is concentration dependent. We found that the higher concentrations of ferrous iron (II) increase the inactivation of CD45, LAR and PTP1B phosphatase caused by hydrogen peroxide, but the addition of the physiological concentration (500 nM) of ferrous iron (II) has even a slightly preventive effect on the phosphatase activity against hydrogen peroxide. Springer Netherlands 2015-09-25 2015 /pmc/articles/PMC4635172/ /pubmed/26407665 http://dx.doi.org/10.1007/s10534-015-9882-4 Text en © The Author(s) 2015 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
spellingShingle Article
Kuban-Jankowska, Alicja
Gorska, Magdalena
Jaremko, Lukasz
Jaremko, Mariusz
Tuszynski, Jack A.
Wozniak, Michal
The physiological concentration of ferrous iron (II) alters the inhibitory effect of hydrogen peroxide on CD45, LAR and PTP1B phosphatases
title The physiological concentration of ferrous iron (II) alters the inhibitory effect of hydrogen peroxide on CD45, LAR and PTP1B phosphatases
title_full The physiological concentration of ferrous iron (II) alters the inhibitory effect of hydrogen peroxide on CD45, LAR and PTP1B phosphatases
title_fullStr The physiological concentration of ferrous iron (II) alters the inhibitory effect of hydrogen peroxide on CD45, LAR and PTP1B phosphatases
title_full_unstemmed The physiological concentration of ferrous iron (II) alters the inhibitory effect of hydrogen peroxide on CD45, LAR and PTP1B phosphatases
title_short The physiological concentration of ferrous iron (II) alters the inhibitory effect of hydrogen peroxide on CD45, LAR and PTP1B phosphatases
title_sort physiological concentration of ferrous iron (ii) alters the inhibitory effect of hydrogen peroxide on cd45, lar and ptp1b phosphatases
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4635172/
https://www.ncbi.nlm.nih.gov/pubmed/26407665
http://dx.doi.org/10.1007/s10534-015-9882-4
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