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mRNA analysis of intracytoplasmically-stained, FACS-purified pancreatic islet cells using the quantitative nuclease protection assay
Exploring the pathophysiology underlying diabetes mellitus requires characterizing the cellular constituents of pancreatic islets, primarily insulin-producing β-cells. Such efforts have been limited by inadequate techniques for purifying islet cellular subsets for further biochemical and gene-expres...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
2009
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4638177/ https://www.ncbi.nlm.nih.gov/pubmed/19838197 http://dx.doi.org/10.1038/nbt.1579 |