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mRNA analysis of intracytoplasmically-stained, FACS-purified pancreatic islet cells using the quantitative nuclease protection assay

Exploring the pathophysiology underlying diabetes mellitus requires characterizing the cellular constituents of pancreatic islets, primarily insulin-producing β-cells. Such efforts have been limited by inadequate techniques for purifying islet cellular subsets for further biochemical and gene-expres...

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Detalles Bibliográficos
Autores principales: Pechhold, S, Stouffer, M, Walker, G, Martel, R, Seligmann, B, Hang, Y, Stein, R, Harlan, DM, Pechhold, K
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2009
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4638177/
https://www.ncbi.nlm.nih.gov/pubmed/19838197
http://dx.doi.org/10.1038/nbt.1579