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Depth-of-Focus Correction in Single-Molecule Data Allows Analysis of 3D Diffusion of the Glucocorticoid Receptor in the Nucleus

Single-molecule imaging of proteins in a 2D environment like membranes has been frequently used to extract diffusive properties of multiple fractions of receptors. In a 3D environment the apparent fractions however change with observation time due to the movements of molecules out of the depth-of-fi...

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Detalles Bibliográficos
Autores principales: Harkes, Rolf, Keizer, Veer I. P., Schaaf, Marcel J. M., Schmidt, Thomas
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4640500/
https://www.ncbi.nlm.nih.gov/pubmed/26555072
http://dx.doi.org/10.1371/journal.pone.0141080