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Depth-of-Focus Correction in Single-Molecule Data Allows Analysis of 3D Diffusion of the Glucocorticoid Receptor in the Nucleus
Single-molecule imaging of proteins in a 2D environment like membranes has been frequently used to extract diffusive properties of multiple fractions of receptors. In a 3D environment the apparent fractions however change with observation time due to the movements of molecules out of the depth-of-fi...
Autores principales: | Harkes, Rolf, Keizer, Veer I. P., Schaaf, Marcel J. M., Schmidt, Thomas |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4640500/ https://www.ncbi.nlm.nih.gov/pubmed/26555072 http://dx.doi.org/10.1371/journal.pone.0141080 |
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