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Use of next generation sequencing data to develop a qPCR method for specific detection of EU-unauthorized genetically modified Bacillus subtilis overproducing riboflavin

BACKGROUND: Recently, the presence of an unauthorized genetically modified (GM) Bacillus subtilis bacterium overproducing vitamin B2 in a feed additive was notified by the Rapid Alert System for Food and Feed (RASFF). This has demonstrated that a contamination by a GM micro-organism (GMM) may occur...

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Detalles Bibliográficos
Autores principales: Barbau-piednoir, Elodie, De Keersmaecker, Sigrid C. J., Delvoye, Maud, Gau, Céline, Philipp, Patrick, Roosens, Nancy H.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4642757/
https://www.ncbi.nlm.nih.gov/pubmed/26559764
http://dx.doi.org/10.1186/s12896-015-0216-y