Accurate label-free reaction kinetics determination using initial rate heat measurements

Accurate label-free methods or assays to obtain the initial reaction rates have significant importance in fundamental studies of enzymes and in application-oriented high throughput screening of enzyme activity. Here we introduce a label-free approach for obtaining initial rates of enzyme activity fr...

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Detalles Bibliográficos
Autores principales: Ebrahimi, Kourosh Honarmand, Hagedoorn, Peter-Leon, Jacobs, Denise, Hagen, Wilfred R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2015
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4647221/
https://www.ncbi.nlm.nih.gov/pubmed/26574737
http://dx.doi.org/10.1038/srep16380

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4647221/
https://www.ncbi.nlm.nih.gov/pubmed/26574737
http://dx.doi.org/10.1038/srep16380

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