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Accurate label-free reaction kinetics determination using initial rate heat measurements
Accurate label-free methods or assays to obtain the initial reaction rates have significant importance in fundamental studies of enzymes and in application-oriented high throughput screening of enzyme activity. Here we introduce a label-free approach for obtaining initial rates of enzyme activity fr...
Autores principales: | Ebrahimi, Kourosh Honarmand, Hagedoorn, Peter-Leon, Jacobs, Denise, Hagen, Wilfred R. |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4647221/ https://www.ncbi.nlm.nih.gov/pubmed/26574737 http://dx.doi.org/10.1038/srep16380 |
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