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Reinitiation at non-canonical start codons leads to leak expression when incorporating unnatural amino acids
With the rapid development of a continuously growing selection of unnatural amino acids (UAAs), UAA insertion becomes increasingly popular for investigating proteins. However, it can prove problematic to ensure the homogeneity of the expressed proteins, when homogeneity is compromised by “leak expre...
| Autores principales: | , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
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Nature Publishing Group
2015
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4648390/ https://www.ncbi.nlm.nih.gov/pubmed/26153354 http://dx.doi.org/10.1038/srep11866 |