Cloning-free CRISPR

We present self-cloning CRISPR/Cas9 (scCRISPR), a technology that allows for CRISPR/Cas9-mediated genomic mutation and site-specific knockin transgene creation within several hours by circumventing the need to clone a site-specific single-guide RNA (sgRNA) or knockin homology construct for each targ...

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Detalles Bibliográficos
Autores principales: Arbab, Mandana, Srinivasan, Sharanya, Hashimoto, Tatsunori, Geijsen, Niels, Sherwood, Richard I.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2015
Materias:
Resource
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4649464/
https://www.ncbi.nlm.nih.gov/pubmed/26527385
http://dx.doi.org/10.1016/j.stemcr.2015.09.022

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4649464/
https://www.ncbi.nlm.nih.gov/pubmed/26527385
http://dx.doi.org/10.1016/j.stemcr.2015.09.022

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