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MicroRNA expression signature of castration-resistant prostate cancer: the microRNA-221/222 cluster functions as a tumour suppressor and disease progression marker
BACKGROUND: Our present study of the microRNA (miRNA) expression signature in castration-resistant prostate cancer (CRPC) revealed that the clustered miRNAs microRNA-221 (miR-221) and microRNA-222 (miR-222) are significantly downregulated in cancer tissues. The aim of this study was to investigate t...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4651127/ https://www.ncbi.nlm.nih.gov/pubmed/26325107 http://dx.doi.org/10.1038/bjc.2015.300 |
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author | Goto, Yusuke Kojima, Satoko Nishikawa, Rika Kurozumi, Akira Kato, Mayuko Enokida, Hideki Matsushita, Ryosuke Yamazaki, Kazuto Ishida, Yasuo Nakagawa, Masayuki Naya, Yukio Ichikawa, Tomohiko Seki, Naohiko |
author_facet | Goto, Yusuke Kojima, Satoko Nishikawa, Rika Kurozumi, Akira Kato, Mayuko Enokida, Hideki Matsushita, Ryosuke Yamazaki, Kazuto Ishida, Yasuo Nakagawa, Masayuki Naya, Yukio Ichikawa, Tomohiko Seki, Naohiko |
author_sort | Goto, Yusuke |
collection | PubMed |
description | BACKGROUND: Our present study of the microRNA (miRNA) expression signature in castration-resistant prostate cancer (CRPC) revealed that the clustered miRNAs microRNA-221 (miR-221) and microRNA-222 (miR-222) are significantly downregulated in cancer tissues. The aim of this study was to investigate the functional roles of miR-221 and miR-222 in prostate cancer (PCa) cells. METHODS: A CRPC miRNA signature was constructed by PCR-based array methods. Functional studies of differentially expressed miRNAs were analysed using PCa cells. The association between miRNA expression and overall survival was estimated by the Kaplan–Meier method. In silico database and genome-wide gene expression analyses were performed to identify molecular targets regulated by the miR-221/222 cluster. RESULTS: miR-221 and miR-222 were significantly downregulated in PCa and CRPC specimens. Kaplan–Meier survival curves showed that low expression of miR-222 predicted a short duration of progression to CRPC. Restoration of miR-221 or miR-222 in cancer cells revealed that both miRNAs significantly inhibited cancer cell migration and invasion. Ecm29 was directly regulated by the miR-221/222 cluster in PCa cells. CONCLUSIONS: Loss of the tumour-suppressive miR-221/222 cluster enhanced migration and invasion in PCa cells. Our data describing targets regulated by the tumour-suppressive miR-221/222 cluster provide insights into the mechanisms of PCa and CRPC progression. |
format | Online Article Text |
id | pubmed-4651127 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-46511272016-09-29 MicroRNA expression signature of castration-resistant prostate cancer: the microRNA-221/222 cluster functions as a tumour suppressor and disease progression marker Goto, Yusuke Kojima, Satoko Nishikawa, Rika Kurozumi, Akira Kato, Mayuko Enokida, Hideki Matsushita, Ryosuke Yamazaki, Kazuto Ishida, Yasuo Nakagawa, Masayuki Naya, Yukio Ichikawa, Tomohiko Seki, Naohiko Br J Cancer Molecular Diagnostics BACKGROUND: Our present study of the microRNA (miRNA) expression signature in castration-resistant prostate cancer (CRPC) revealed that the clustered miRNAs microRNA-221 (miR-221) and microRNA-222 (miR-222) are significantly downregulated in cancer tissues. The aim of this study was to investigate the functional roles of miR-221 and miR-222 in prostate cancer (PCa) cells. METHODS: A CRPC miRNA signature was constructed by PCR-based array methods. Functional studies of differentially expressed miRNAs were analysed using PCa cells. The association between miRNA expression and overall survival was estimated by the Kaplan–Meier method. In silico database and genome-wide gene expression analyses were performed to identify molecular targets regulated by the miR-221/222 cluster. RESULTS: miR-221 and miR-222 were significantly downregulated in PCa and CRPC specimens. Kaplan–Meier survival curves showed that low expression of miR-222 predicted a short duration of progression to CRPC. Restoration of miR-221 or miR-222 in cancer cells revealed that both miRNAs significantly inhibited cancer cell migration and invasion. Ecm29 was directly regulated by the miR-221/222 cluster in PCa cells. CONCLUSIONS: Loss of the tumour-suppressive miR-221/222 cluster enhanced migration and invasion in PCa cells. Our data describing targets regulated by the tumour-suppressive miR-221/222 cluster provide insights into the mechanisms of PCa and CRPC progression. Nature Publishing Group 2015-09-29 2015-09-01 /pmc/articles/PMC4651127/ /pubmed/26325107 http://dx.doi.org/10.1038/bjc.2015.300 Text en Copyright © 2015 Cancer Research UK http://creativecommons.org/licenses/by-nc-sa/4.0/ From twelve months after its original publication, this work is licensed under the Creative Commons Attribution-NonCommercial-Share Alike 4.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-sa/4.0/ |
spellingShingle | Molecular Diagnostics Goto, Yusuke Kojima, Satoko Nishikawa, Rika Kurozumi, Akira Kato, Mayuko Enokida, Hideki Matsushita, Ryosuke Yamazaki, Kazuto Ishida, Yasuo Nakagawa, Masayuki Naya, Yukio Ichikawa, Tomohiko Seki, Naohiko MicroRNA expression signature of castration-resistant prostate cancer: the microRNA-221/222 cluster functions as a tumour suppressor and disease progression marker |
title | MicroRNA expression signature of castration-resistant prostate cancer: the microRNA-221/222 cluster functions as a tumour suppressor and disease progression marker |
title_full | MicroRNA expression signature of castration-resistant prostate cancer: the microRNA-221/222 cluster functions as a tumour suppressor and disease progression marker |
title_fullStr | MicroRNA expression signature of castration-resistant prostate cancer: the microRNA-221/222 cluster functions as a tumour suppressor and disease progression marker |
title_full_unstemmed | MicroRNA expression signature of castration-resistant prostate cancer: the microRNA-221/222 cluster functions as a tumour suppressor and disease progression marker |
title_short | MicroRNA expression signature of castration-resistant prostate cancer: the microRNA-221/222 cluster functions as a tumour suppressor and disease progression marker |
title_sort | microrna expression signature of castration-resistant prostate cancer: the microrna-221/222 cluster functions as a tumour suppressor and disease progression marker |
topic | Molecular Diagnostics |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4651127/ https://www.ncbi.nlm.nih.gov/pubmed/26325107 http://dx.doi.org/10.1038/bjc.2015.300 |
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