The human decapping scavenger enzyme DcpS modulates microRNA turnover

The decapping scavenger enzyme DcpS is known for its role in hydrolyzing the cap structure following mRNA degradation. Recently, we discovered a new function in miRNA degradation activation for the ortholog of DcpS in C. elegans. Here we show that human DcpS conserves its role in miRNA turnover. In...

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Autores principales: Meziane, Oussama, Piquet, Sandra, Bossé, Gabriel D., Gagné, Dominic, Paquet, Eric, Robert, Claude, Tones, Michael A., Simard, Martin J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2015
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4653633/
https://www.ncbi.nlm.nih.gov/pubmed/26584588
http://dx.doi.org/10.1038/srep16688
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author Meziane, Oussama
Piquet, Sandra
Bossé, Gabriel D.
Gagné, Dominic
Paquet, Eric
Robert, Claude
Tones, Michael A.
Simard, Martin J.
author_facet Meziane, Oussama
Piquet, Sandra
Bossé, Gabriel D.
Gagné, Dominic
Paquet, Eric
Robert, Claude
Tones, Michael A.
Simard, Martin J.
author_sort Meziane, Oussama
collection PubMed
description The decapping scavenger enzyme DcpS is known for its role in hydrolyzing the cap structure following mRNA degradation. Recently, we discovered a new function in miRNA degradation activation for the ortholog of DcpS in C. elegans. Here we show that human DcpS conserves its role in miRNA turnover. In human cells, DcpS is a nucleocytoplasmic shuttling protein that activates miRNA degradation independently of its scavenger decapping activity in the cytoplasmic compartment. We also demonstrate that this new function for DcpS requires the contribution of the 5′-3′ exonuclease Xrn2. Our findings support a conserved role of DcpS as a modulator of miRNA turnover in animals.
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spelling pubmed-46536332015-11-25 The human decapping scavenger enzyme DcpS modulates microRNA turnover Meziane, Oussama Piquet, Sandra Bossé, Gabriel D. Gagné, Dominic Paquet, Eric Robert, Claude Tones, Michael A. Simard, Martin J. Sci Rep Article The decapping scavenger enzyme DcpS is known for its role in hydrolyzing the cap structure following mRNA degradation. Recently, we discovered a new function in miRNA degradation activation for the ortholog of DcpS in C. elegans. Here we show that human DcpS conserves its role in miRNA turnover. In human cells, DcpS is a nucleocytoplasmic shuttling protein that activates miRNA degradation independently of its scavenger decapping activity in the cytoplasmic compartment. We also demonstrate that this new function for DcpS requires the contribution of the 5′-3′ exonuclease Xrn2. Our findings support a conserved role of DcpS as a modulator of miRNA turnover in animals. Nature Publishing Group 2015-11-20 /pmc/articles/PMC4653633/ /pubmed/26584588 http://dx.doi.org/10.1038/srep16688 Text en Copyright © 2015, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Meziane, Oussama
Piquet, Sandra
Bossé, Gabriel D.
Gagné, Dominic
Paquet, Eric
Robert, Claude
Tones, Michael A.
Simard, Martin J.
The human decapping scavenger enzyme DcpS modulates microRNA turnover
title The human decapping scavenger enzyme DcpS modulates microRNA turnover
title_full The human decapping scavenger enzyme DcpS modulates microRNA turnover
title_fullStr The human decapping scavenger enzyme DcpS modulates microRNA turnover
title_full_unstemmed The human decapping scavenger enzyme DcpS modulates microRNA turnover
title_short The human decapping scavenger enzyme DcpS modulates microRNA turnover
title_sort human decapping scavenger enzyme dcps modulates microrna turnover
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4653633/
https://www.ncbi.nlm.nih.gov/pubmed/26584588
http://dx.doi.org/10.1038/srep16688
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