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Questioned validity of Gene Expression Dysregulated Domains in Down's Syndrome
Recently, in studies examining fibroblasts obtained from the tissues of one set of monozygotic twins (i.e. fetuses derived from the same egg) discordant for trisomy 21 (Down syndrome; DS), Letourneau et al., ( )reported the presence of a defined pattern of dysregulation within specific genomic domai...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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F1000Research
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4654439/ https://www.ncbi.nlm.nih.gov/pubmed/26664707 http://dx.doi.org/10.12688/f1000research.6735.1 |
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author | Do, Long H. Mobley, William C. Singhal, Nishant |
author_facet | Do, Long H. Mobley, William C. Singhal, Nishant |
author_sort | Do, Long H. |
collection | PubMed |
description | Recently, in studies examining fibroblasts obtained from the tissues of one set of monozygotic twins (i.e. fetuses derived from the same egg) discordant for trisomy 21 (Down syndrome; DS), Letourneau et al., ( )reported the presence of a defined pattern of dysregulation within specific genomic domains they referred to as Gene Expression Dysregulated Domains (GEDDs). GEDDs were described as alternating segments of increased or decreased gene expression affecting all chromosomes. Strikingly, GEDDs in fibroblasts were largely conserved in induced pluripotent cells (iPSCs) generated from the twin’s fibroblasts as well as in fibroblasts from the Ts65Dn mouse model of DS. Our recent analysis failed to find GEDDs. We reexamined the human iPSCs RNAseq data from Letourneau et al., and data from this same research group published earlier examining iPSCs from the same monozygotic twins. An independent analysis of RNAseq data from Ts65Dn fibroblasts also failed to confirm presence of GEDDs. Our analysis questions the validity of GEDDs in DS. |
format | Online Article Text |
id | pubmed-4654439 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | F1000Research |
record_format | MEDLINE/PubMed |
spelling | pubmed-46544392015-12-09 Questioned validity of Gene Expression Dysregulated Domains in Down's Syndrome Do, Long H. Mobley, William C. Singhal, Nishant F1000Res Research Note Recently, in studies examining fibroblasts obtained from the tissues of one set of monozygotic twins (i.e. fetuses derived from the same egg) discordant for trisomy 21 (Down syndrome; DS), Letourneau et al., ( )reported the presence of a defined pattern of dysregulation within specific genomic domains they referred to as Gene Expression Dysregulated Domains (GEDDs). GEDDs were described as alternating segments of increased or decreased gene expression affecting all chromosomes. Strikingly, GEDDs in fibroblasts were largely conserved in induced pluripotent cells (iPSCs) generated from the twin’s fibroblasts as well as in fibroblasts from the Ts65Dn mouse model of DS. Our recent analysis failed to find GEDDs. We reexamined the human iPSCs RNAseq data from Letourneau et al., and data from this same research group published earlier examining iPSCs from the same monozygotic twins. An independent analysis of RNAseq data from Ts65Dn fibroblasts also failed to confirm presence of GEDDs. Our analysis questions the validity of GEDDs in DS. F1000Research 2015-07-17 /pmc/articles/PMC4654439/ /pubmed/26664707 http://dx.doi.org/10.12688/f1000research.6735.1 Text en Copyright: © 2015 Do LH et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Note Do, Long H. Mobley, William C. Singhal, Nishant Questioned validity of Gene Expression Dysregulated Domains in Down's Syndrome |
title | Questioned validity of Gene Expression Dysregulated Domains in Down's Syndrome |
title_full | Questioned validity of Gene Expression Dysregulated Domains in Down's Syndrome |
title_fullStr | Questioned validity of Gene Expression Dysregulated Domains in Down's Syndrome |
title_full_unstemmed | Questioned validity of Gene Expression Dysregulated Domains in Down's Syndrome |
title_short | Questioned validity of Gene Expression Dysregulated Domains in Down's Syndrome |
title_sort | questioned validity of gene expression dysregulated domains in down's syndrome |
topic | Research Note |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4654439/ https://www.ncbi.nlm.nih.gov/pubmed/26664707 http://dx.doi.org/10.12688/f1000research.6735.1 |
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