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The Structure of a Novel Thermophilic Esterase from the Planctomycetes Species, Thermogutta terrifontis Reveals an Open Active Site Due to a Minimal ‘Cap’ Domain

A carboxyl esterase (TtEst2) has been identified in a novel thermophilic bacterium, Thermogutta terrifontis from the phylum Planctomycetes and has been cloned and over-expressed in Escherichia coli. The enzyme has been characterized biochemically and shown to have activity toward small p-nitrophenyl...

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Detalles Bibliográficos
Autores principales: Sayer, Christopher, Szabo, Zalan, Isupov, Michail N., Ingham, Colin, Littlechild, Jennifer A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4655241/
https://www.ncbi.nlm.nih.gov/pubmed/26635762
http://dx.doi.org/10.3389/fmicb.2015.01294