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A high-density immunoblotting methodology for quantification of total protein levels and phosphorylation modifications
The components of many signaling pathways have been identified and there is now a need to conduct quantitative data-rich temporal experiments for systems biology and modeling approaches to better understand pathway dynamics and regulation. Here we present a modified Western blotting method that allo...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4655314/ https://www.ncbi.nlm.nih.gov/pubmed/26592927 http://dx.doi.org/10.1038/srep16995 |