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A high-density immunoblotting methodology for quantification of total protein levels and phosphorylation modifications

The components of many signaling pathways have been identified and there is now a need to conduct quantitative data-rich temporal experiments for systems biology and modeling approaches to better understand pathway dynamics and regulation. Here we present a modified Western blotting method that allo...

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Detalles Bibliográficos
Autores principales: Mazet, F., Dunster, J. L., Jones, C. I., Vaiyapuri, S., Tindall, M. J., Fry, M. J., Gibbins, J. M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4655314/
https://www.ncbi.nlm.nih.gov/pubmed/26592927
http://dx.doi.org/10.1038/srep16995