Direct uptake of Antagomirs and efficient knockdown of miRNA in primary B and T lymphocytes

Depending on their physiological expression level, microRNAs (miRNA) address different target genes, thus have different biological functions. To identify these, the physiological expression has to be blocked. Here, we describe the use of inhibitory cholesterol-modified oligonucleotides (Antagomirs)...

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Detalles Bibliográficos
Autores principales: Haftmann, Claudia, Riedel, René, Porstner, Martina, Wittmann, Jürgen, Chang, Hyun-Dong, Radbruch, Andreas, Mashreghi, Mir-Farzin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2015
Materias:
Technical Note
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4655414/
https://www.ncbi.nlm.nih.gov/pubmed/26187895
http://dx.doi.org/10.1016/j.jim.2015.07.006
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author Haftmann, Claudia
Riedel, René
Porstner, Martina
Wittmann, Jürgen
Chang, Hyun-Dong
Radbruch, Andreas
Mashreghi, Mir-Farzin
author_facet Haftmann, Claudia
Riedel, René
Porstner, Martina
Wittmann, Jürgen
Chang, Hyun-Dong
Radbruch, Andreas
Mashreghi, Mir-Farzin
author_sort Haftmann, Claudia
collection PubMed
description Depending on their physiological expression level, microRNAs (miRNA) address different target genes, thus have different biological functions. To identify these, the physiological expression has to be blocked. Here, we describe the use of inhibitory cholesterol-modified oligonucleotides (Antagomirs) to inhibit miRNAs selectively in primary human and murine T and B lymphocytes. Due to their lipophilic cholesterol tag Antagomirs enter primary lymphocytes efficiently and quantitatively. We show here that at concentrations of 0.125 to 1 μM, Antagomirs selectively inhibit expression of their target miRNA up to 99.5% without affecting cell viability.
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spelling pubmed-46554142015-12-18 Direct uptake of Antagomirs and efficient knockdown of miRNA in primary B and T lymphocytes Haftmann, Claudia Riedel, René Porstner, Martina Wittmann, Jürgen Chang, Hyun-Dong Radbruch, Andreas Mashreghi, Mir-Farzin J Immunol Methods Technical Note Depending on their physiological expression level, microRNAs (miRNA) address different target genes, thus have different biological functions. To identify these, the physiological expression has to be blocked. Here, we describe the use of inhibitory cholesterol-modified oligonucleotides (Antagomirs) to inhibit miRNAs selectively in primary human and murine T and B lymphocytes. Due to their lipophilic cholesterol tag Antagomirs enter primary lymphocytes efficiently and quantitatively. We show here that at concentrations of 0.125 to 1 μM, Antagomirs selectively inhibit expression of their target miRNA up to 99.5% without affecting cell viability. Elsevier 2015-11 /pmc/articles/PMC4655414/ /pubmed/26187895 http://dx.doi.org/10.1016/j.jim.2015.07.006 Text en © 2015 The Authors. Published by Elsevier B.V. http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Technical Note
Haftmann, Claudia
Riedel, René
Porstner, Martina
Wittmann, Jürgen
Chang, Hyun-Dong
Radbruch, Andreas
Mashreghi, Mir-Farzin
Direct uptake of Antagomirs and efficient knockdown of miRNA in primary B and T lymphocytes
title Direct uptake of Antagomirs and efficient knockdown of miRNA in primary B and T lymphocytes
title_full Direct uptake of Antagomirs and efficient knockdown of miRNA in primary B and T lymphocytes
title_fullStr Direct uptake of Antagomirs and efficient knockdown of miRNA in primary B and T lymphocytes
title_full_unstemmed Direct uptake of Antagomirs and efficient knockdown of miRNA in primary B and T lymphocytes
title_short Direct uptake of Antagomirs and efficient knockdown of miRNA in primary B and T lymphocytes
title_sort direct uptake of antagomirs and efficient knockdown of mirna in primary b and t lymphocytes
topic Technical Note
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4655414/
https://www.ncbi.nlm.nih.gov/pubmed/26187895
http://dx.doi.org/10.1016/j.jim.2015.07.006
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