Extracting Cell Stiffness from Real-Time Deformability Cytometry: Theory and Experiment

Cell stiffness is a sensitive indicator of physiological and pathological changes in cells, with many potential applications in biology and medicine. A new method, real-time deformability cytometry, probes cell stiffness at high throughput by exposing cells to a shear flow in a microfluidic channel,...

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Autores principales: Mietke, Alexander, Otto, Oliver, Girardo, Salvatore, Rosendahl, Philipp, Taubenberger, Anna, Golfier, Stefan, Ulbricht, Elke, Aland, Sebastian, Guck, Jochen, Fischer-Friedrich, Elisabeth
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Biophysical Society 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4656812/
https://www.ncbi.nlm.nih.gov/pubmed/26588562
http://dx.doi.org/10.1016/j.bpj.2015.09.006
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author Mietke, Alexander
Otto, Oliver
Girardo, Salvatore
Rosendahl, Philipp
Taubenberger, Anna
Golfier, Stefan
Ulbricht, Elke
Aland, Sebastian
Guck, Jochen
Fischer-Friedrich, Elisabeth
author_facet Mietke, Alexander
Otto, Oliver
Girardo, Salvatore
Rosendahl, Philipp
Taubenberger, Anna
Golfier, Stefan
Ulbricht, Elke
Aland, Sebastian
Guck, Jochen
Fischer-Friedrich, Elisabeth
author_sort Mietke, Alexander
collection PubMed
description Cell stiffness is a sensitive indicator of physiological and pathological changes in cells, with many potential applications in biology and medicine. A new method, real-time deformability cytometry, probes cell stiffness at high throughput by exposing cells to a shear flow in a microfluidic channel, allowing for mechanical phenotyping based on single-cell deformability. However, observed deformations of cells in the channel not only are determined by cell stiffness, but also depend on cell size relative to channel size. Here, we disentangle mutual contributions of cell size and cell stiffness to cell deformation by a theoretical analysis in terms of hydrodynamics and linear elasticity theory. Performing real-time deformability cytometry experiments on both model spheres of known elasticity and biological cells, we demonstrate that our analytical model not only predicts deformed shapes inside the channel but also allows for quantification of cell mechanical parameters. Thereby, fast and quantitative mechanical sampling of large cell populations becomes feasible.
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spelling pubmed-46568122016-11-17 Extracting Cell Stiffness from Real-Time Deformability Cytometry: Theory and Experiment Mietke, Alexander Otto, Oliver Girardo, Salvatore Rosendahl, Philipp Taubenberger, Anna Golfier, Stefan Ulbricht, Elke Aland, Sebastian Guck, Jochen Fischer-Friedrich, Elisabeth Biophys J Cell Biophysics Cell stiffness is a sensitive indicator of physiological and pathological changes in cells, with many potential applications in biology and medicine. A new method, real-time deformability cytometry, probes cell stiffness at high throughput by exposing cells to a shear flow in a microfluidic channel, allowing for mechanical phenotyping based on single-cell deformability. However, observed deformations of cells in the channel not only are determined by cell stiffness, but also depend on cell size relative to channel size. Here, we disentangle mutual contributions of cell size and cell stiffness to cell deformation by a theoretical analysis in terms of hydrodynamics and linear elasticity theory. Performing real-time deformability cytometry experiments on both model spheres of known elasticity and biological cells, we demonstrate that our analytical model not only predicts deformed shapes inside the channel but also allows for quantification of cell mechanical parameters. Thereby, fast and quantitative mechanical sampling of large cell populations becomes feasible. The Biophysical Society 2015-11-17 2015-11-17 /pmc/articles/PMC4656812/ /pubmed/26588562 http://dx.doi.org/10.1016/j.bpj.2015.09.006 Text en © 2015 The Authors http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Cell Biophysics
Mietke, Alexander
Otto, Oliver
Girardo, Salvatore
Rosendahl, Philipp
Taubenberger, Anna
Golfier, Stefan
Ulbricht, Elke
Aland, Sebastian
Guck, Jochen
Fischer-Friedrich, Elisabeth
Extracting Cell Stiffness from Real-Time Deformability Cytometry: Theory and Experiment
title Extracting Cell Stiffness from Real-Time Deformability Cytometry: Theory and Experiment
title_full Extracting Cell Stiffness from Real-Time Deformability Cytometry: Theory and Experiment
title_fullStr Extracting Cell Stiffness from Real-Time Deformability Cytometry: Theory and Experiment
title_full_unstemmed Extracting Cell Stiffness from Real-Time Deformability Cytometry: Theory and Experiment
title_short Extracting Cell Stiffness from Real-Time Deformability Cytometry: Theory and Experiment
title_sort extracting cell stiffness from real-time deformability cytometry: theory and experiment
topic Cell Biophysics
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4656812/
https://www.ncbi.nlm.nih.gov/pubmed/26588562
http://dx.doi.org/10.1016/j.bpj.2015.09.006
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