In vivo polyester immobilized sortase for tagless protein purification

BACKGROUND: Laboratory scale recombinant protein production and purification techniques are often complicated, involving multiple chromatography steps and specialized equipment and reagents. Here it was demonstrated that recombinant proteins can be expressed as covalently immobilized to the surface...

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Detalles Bibliográficos
Autores principales: Hay, Iain D., Du, Jinping, Reyes, Patricia Rubio, Rehm, Bernd H. A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2015
Materias:
Technical Notes
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4658790/
https://www.ncbi.nlm.nih.gov/pubmed/26608345
http://dx.doi.org/10.1186/s12934-015-0385-3

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4658790/
https://www.ncbi.nlm.nih.gov/pubmed/26608345
http://dx.doi.org/10.1186/s12934-015-0385-3

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