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Understanding the link between single cell and population scale responses of Escherichia coli in differing ligand gradients

We formulate an agent-based population model of Escherichia coli cells which incorporates a description of the chemotaxis signalling cascade at the single cell scale. The model is used to gain insight into the link between the signalling cascade dynamics and the overall population response to differ...

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Detalles Bibliográficos
Autores principales: Edgington, Matthew P., Tindall, Marcus J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Research Network of Computational and Structural Biotechnology 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4660157/
https://www.ncbi.nlm.nih.gov/pubmed/26693274
http://dx.doi.org/10.1016/j.csbj.2015.09.003
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author Edgington, Matthew P.
Tindall, Marcus J.
author_facet Edgington, Matthew P.
Tindall, Marcus J.
author_sort Edgington, Matthew P.
collection PubMed
description We formulate an agent-based population model of Escherichia coli cells which incorporates a description of the chemotaxis signalling cascade at the single cell scale. The model is used to gain insight into the link between the signalling cascade dynamics and the overall population response to differing chemoattractant gradients. Firstly, we consider how the observed variation in total (phosphorylated and unphosphorylated) signalling protein concentration affects the ability of cells to accumulate in differing chemoattractant gradients. Results reveal that a variation in total cell protein concentration between cells may be a mechanism for the survival of cell colonies across a wide range of differing environments. We then study the response of cells in the presence of two different chemoattractants. In doing so we demonstrate that the population scale response depends not on the absolute concentration of each chemoattractant but on the sensitivity of the chemoreceptors to their respective concentrations. Our results show the clear link between single cell features and the overall environment in which cells reside.
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spelling pubmed-46601572015-12-21 Understanding the link between single cell and population scale responses of Escherichia coli in differing ligand gradients Edgington, Matthew P. Tindall, Marcus J. Comput Struct Biotechnol J Research Article We formulate an agent-based population model of Escherichia coli cells which incorporates a description of the chemotaxis signalling cascade at the single cell scale. The model is used to gain insight into the link between the signalling cascade dynamics and the overall population response to differing chemoattractant gradients. Firstly, we consider how the observed variation in total (phosphorylated and unphosphorylated) signalling protein concentration affects the ability of cells to accumulate in differing chemoattractant gradients. Results reveal that a variation in total cell protein concentration between cells may be a mechanism for the survival of cell colonies across a wide range of differing environments. We then study the response of cells in the presence of two different chemoattractants. In doing so we demonstrate that the population scale response depends not on the absolute concentration of each chemoattractant but on the sensitivity of the chemoreceptors to their respective concentrations. Our results show the clear link between single cell features and the overall environment in which cells reside. Research Network of Computational and Structural Biotechnology 2015-10-26 /pmc/articles/PMC4660157/ /pubmed/26693274 http://dx.doi.org/10.1016/j.csbj.2015.09.003 Text en © 2015 The Authors http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Research Article
Edgington, Matthew P.
Tindall, Marcus J.
Understanding the link between single cell and population scale responses of Escherichia coli in differing ligand gradients
title Understanding the link between single cell and population scale responses of Escherichia coli in differing ligand gradients
title_full Understanding the link between single cell and population scale responses of Escherichia coli in differing ligand gradients
title_fullStr Understanding the link between single cell and population scale responses of Escherichia coli in differing ligand gradients
title_full_unstemmed Understanding the link between single cell and population scale responses of Escherichia coli in differing ligand gradients
title_short Understanding the link between single cell and population scale responses of Escherichia coli in differing ligand gradients
title_sort understanding the link between single cell and population scale responses of escherichia coli in differing ligand gradients
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4660157/
https://www.ncbi.nlm.nih.gov/pubmed/26693274
http://dx.doi.org/10.1016/j.csbj.2015.09.003
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