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Construction of a high-density mutant library in soybean and development of a mutant retrieval method using amplicon sequencing

BACKGROUND: Functions of most genes predicted in the soybean genome have not been clarified. A mutant library with a high mutation density would be helpful for functional studies and for identification of novel alleles useful for breeding. Development of cost-effective and high-throughput protocols...

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Detalles Bibliográficos
Autores principales: Tsuda, Mai, Kaga, Akito, Anai, Toyoaki, Shimizu, Takehiko, Sayama, Takashi, Takagi, Kyoko, Machita, Kayo, Watanabe, Satoshi, Nishimura, Minoru, Yamada, Naohiro, Mori, Satomi, Sasaki, Harumi, Kanamori, Hiroyuki, Katayose, Yuichi, Ishimoto, Masao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4662035/
https://www.ncbi.nlm.nih.gov/pubmed/26610706
http://dx.doi.org/10.1186/s12864-015-2079-y