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Comparison Study on Changes of Antigenicities of Egg Ovalbumin Irradiated by Electron Beam or X-Ray

This study was conducted to compare the effects of two forms of radiation (electron and X-ray; generated by an electron beam accelerator) on the conformation and antigenic properties of hen’s egg albumin, ovalbumin (OVA), which was used as a model protein. OVA solutions (2.0 mg/mL) were individually...

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Autores principales: Kim, Mi-Jung, Lee, Ju-Woon, Sung, Nak-Yoon, Kim, Su-Min, Hwang, Young-Jung, Kim, Jae-Hun, Song, Beom-Seok
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Korean Society for Food Science of Animal Resources 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4662217/
https://www.ncbi.nlm.nih.gov/pubmed/26761489
http://dx.doi.org/10.5851/kosfa.2014.34.5.570
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author Kim, Mi-Jung
Lee, Ju-Woon
Sung, Nak-Yoon
Kim, Su-Min
Hwang, Young-Jung
Kim, Jae-Hun
Song, Beom-Seok
author_facet Kim, Mi-Jung
Lee, Ju-Woon
Sung, Nak-Yoon
Kim, Su-Min
Hwang, Young-Jung
Kim, Jae-Hun
Song, Beom-Seok
author_sort Kim, Mi-Jung
collection PubMed
description This study was conducted to compare the effects of two forms of radiation (electron and X-ray; generated by an electron beam accelerator) on the conformation and antigenic properties of hen’s egg albumin, ovalbumin (OVA), which was used as a model protein. OVA solutions (2.0 mg/mL) were individually irradiated by electron beam or X-ray at the absorbed doses of 0 (control), 2, 4, 6, 8, and 10 kGy. No differences between the two forms of radiation on the structural properties of OVA were shown by spectrometric and electrophoretic analyses. The turbidity of OVA solution increased and the main OVA bands on polyacrylamide gels disappeared after irradiation, regardless of the radiation source. In competitive indirect enzyme-linked immunosorbent assay, OVA samples irradiated by electron beam or X-ray showed different immunological responses in reactions with monoclonal and polyclonal antibodies (immunoglobulin G) produced against non-irradiated OVA. The results indicate that electron beam irradiation and X-ray irradiation produced different patterns of structural changes to the OVA molecule.
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spelling pubmed-46622172016-01-04 Comparison Study on Changes of Antigenicities of Egg Ovalbumin Irradiated by Electron Beam or X-Ray Kim, Mi-Jung Lee, Ju-Woon Sung, Nak-Yoon Kim, Su-Min Hwang, Young-Jung Kim, Jae-Hun Song, Beom-Seok Korean J Food Sci Anim Resour Article This study was conducted to compare the effects of two forms of radiation (electron and X-ray; generated by an electron beam accelerator) on the conformation and antigenic properties of hen’s egg albumin, ovalbumin (OVA), which was used as a model protein. OVA solutions (2.0 mg/mL) were individually irradiated by electron beam or X-ray at the absorbed doses of 0 (control), 2, 4, 6, 8, and 10 kGy. No differences between the two forms of radiation on the structural properties of OVA were shown by spectrometric and electrophoretic analyses. The turbidity of OVA solution increased and the main OVA bands on polyacrylamide gels disappeared after irradiation, regardless of the radiation source. In competitive indirect enzyme-linked immunosorbent assay, OVA samples irradiated by electron beam or X-ray showed different immunological responses in reactions with monoclonal and polyclonal antibodies (immunoglobulin G) produced against non-irradiated OVA. The results indicate that electron beam irradiation and X-ray irradiation produced different patterns of structural changes to the OVA molecule. Korean Society for Food Science of Animal Resources 2014 2014-10-31 /pmc/articles/PMC4662217/ /pubmed/26761489 http://dx.doi.org/10.5851/kosfa.2014.34.5.570 Text en Copyright © 2014, Korean Society for Food Science of Animal Resources http://creativecommons.org/licences/by-nc/4.0 This is an open access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licences/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Article
Kim, Mi-Jung
Lee, Ju-Woon
Sung, Nak-Yoon
Kim, Su-Min
Hwang, Young-Jung
Kim, Jae-Hun
Song, Beom-Seok
Comparison Study on Changes of Antigenicities of Egg Ovalbumin Irradiated by Electron Beam or X-Ray
title Comparison Study on Changes of Antigenicities of Egg Ovalbumin Irradiated by Electron Beam or X-Ray
title_full Comparison Study on Changes of Antigenicities of Egg Ovalbumin Irradiated by Electron Beam or X-Ray
title_fullStr Comparison Study on Changes of Antigenicities of Egg Ovalbumin Irradiated by Electron Beam or X-Ray
title_full_unstemmed Comparison Study on Changes of Antigenicities of Egg Ovalbumin Irradiated by Electron Beam or X-Ray
title_short Comparison Study on Changes of Antigenicities of Egg Ovalbumin Irradiated by Electron Beam or X-Ray
title_sort comparison study on changes of antigenicities of egg ovalbumin irradiated by electron beam or x-ray
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4662217/
https://www.ncbi.nlm.nih.gov/pubmed/26761489
http://dx.doi.org/10.5851/kosfa.2014.34.5.570
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