Cargando…

Characterisation of aptamer–target interactions by branched selection and high-throughput sequencing of SELEX pools

Nucleic acid aptamer selection by systematic evolution of ligands by exponential enrichment (SELEX) has shown great promise for use in the development of research tools, therapeutics and diagnostics. Typically, aptamers are identified from libraries containing up to 10(16) different RNA or DNA seque...

Descripción completa

Detalles Bibliográficos
Autores principales:	Dupont, Daniel M., Larsen, Niels, Jensen, Jan. K., Andreasen, Peter A., Kjems, Jørgen
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Oxford University Press 2015
Materias:	Methods Online
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4666376/ https://www.ncbi.nlm.nih.gov/pubmed/26163061 http://dx.doi.org/10.1093/nar/gkv700

Ejemplares similares

Selection of allosteric dnazymes that can sense phenylalanine by expression-SELEX
por: Chen, Binfen, et al.
Publicado: (2023)

Characterization of homing endonuclease binding and cleavage specificities using yeast surface display SELEX (YSD-SELEX)
por: Jacoby, Kyle, et al.
Publicado: (2017)

In silico selection of RNA aptamers
por: Chushak, Yaroslav, et al.
Publicado: (2009)

High-throughput quantitative binding analysis of DNA aptamers using exonucleases
por: Canoura, Juan, et al.
Publicado: (2022)

Combining SELEX with quantitative assays to rapidly obtain accurate models of protein–DNA interactions
por: Liu, Jiajian, et al.
Publicado: (2005)

Quantifying selection in high-throughput Immunoglobulin sequencing data sets
por: Yaari, Gur, et al.
Publicado: (2012)

Single-Step Selection of Bivalent Aptamers Validated by Comparison with SELEX Using High-Throughput Sequencing
por: Wilson, Robert, et al.
Publicado: (2014)

Selection of DNA aptamers using atomic force microscopy
por: Miyachi, Yusuke, et al.
Publicado: (2010)

Assessing serotonin receptor mRNA editing frequency by a novel ultra high-throughput sequencing method
por: Abbas, Atheir I., et al.
Publicado: (2010)

Identification of target-binding peptide motifs by high-throughput sequencing of phage-selected peptides
por: Rentero Rebollo, Inmaculada, et al.
Publicado: (2014)

A comparative analysis of transcription factor binding models learned from PBM, HT-SELEX and ChIP data
por: Orenstein, Yaron, et al.
Publicado: (2014)

Differential RNA aptamer affinity profiling on plasma as a potential diagnostic tool for bladder cancer
por: Fjelstrup, Søren, et al.
Publicado: (2022)

Selection of aptamers for a protein target in cell lysate and their application to protein purification
por: Javaherian, Sahar, et al.
Publicado: (2009)

Identifying high-affinity aptamer ligands with defined cross-reactivity using high-throughput guided systematic evolution of ligands by exponential enrichment
por: Levay, Agata, et al.
Publicado: (2015)

Targeting individual cells by barcode in pooled sequence libraries
por: Ranu, Navpreet, et al.
Publicado: (2019)

Selection of DNA aptamers for ovarian cancer biomarker HE4 using CE-SELEX and high-throughput sequencing
por: Eaton, Rachel M., et al.
Publicado: (2015)

Array-based evolution of DNA aptamers allows modelling of an explicit sequence-fitness landscape
por: Knight, Christopher G., et al.
Publicado: (2009)

SMARTer single cell total RNA sequencing
por: Verboom, Karen, et al.
Publicado: (2019)

miRdentify: high stringency miRNA predictor identifies several novel animal miRNAs
por: Hansen, Thomas B., et al.
Publicado: (2014)

Comparison of circular RNA prediction tools
por: Hansen, Thomas B., et al.
Publicado: (2016)

Refining the Results of a Classical SELEX Experiment by Expanding the Sequence Data Set of an Aptamer Pool Selected for Protein A
por: Stoltenburg, Regina, et al.
Publicado: (2018)

An accurate clone-based haplotyping method by overlapping pool sequencing
por: Li, Cheng, et al.
Publicado: (2016)

Highly selective retrieval of accurate DNA utilizing a pool of in situ-replicated DNA from multiple next-generation sequencing platforms
por: Lim, Hyeonseob, et al.
Publicado: (2018)

Computational generation and screening of RNA motifs in large nucleotide sequence pools
por: Kim, Namhee, et al.
Publicado: (2010)

Aptamer mediated siRNA delivery
por: Chu, Ted C., et al.
Publicado: (2006)

Identification of nucleic acid aptamers against lactate dehydrogenase via SELEX and high-throughput sequencing
por: Guo, Linghui, et al.
Publicado: (2021)

Selection of DNA Aptamers for Ovarian Cancer Biomarker CA125 Using One-Pot SELEX and High-Throughput Sequencing
por: Scoville, Delia J., et al.
Publicado: (2017)

RAPID-SELEX for RNA Aptamers
por: Szeto, Kylan, et al.
Publicado: (2013)

Highly-multiplexed barcode sequencing: an efficient method for parallel analysis of pooled samples
por: Smith, Andrew M., et al.
Publicado: (2010)

Capture-SELEX: Selection of DNA Aptamers for Aminoglycoside Antibiotics
por: Stoltenburg, Regina, et al.
Publicado: (2012)

Targeted high-throughput sequencing of tagged nucleic acid samples
por: Meyer, Matthias, et al.
Publicado: (2007)

Summarizing and correcting the GC content bias in high-throughput sequencing
por: Benjamini, Yuval, et al.
Publicado: (2012)

Detecting Alu insertions from high-throughput sequencing data
por: David, Matei, et al.
Publicado: (2013)

High-throughput assay and engineering of self-cleaving ribozymes by sequencing
por: Kobori, Shungo, et al.
Publicado: (2015)

Determination of tRNA aminoacylation levels by high-throughput sequencing
por: Evans, Molly E., et al.
Publicado: (2017)

Using high-throughput barcode sequencing to efficiently map connectomes
por: Peikon, Ian D., et al.
Publicado: (2017)

Composite RNA aptamers as functional mimics of proteins
por: Xu, Daiying, et al.
Publicado: (2009)

Estimate of within population incremental selection through branch imbalance in lineage trees
por: Liberman, Gilad, et al.
Publicado: (2016)

Development of an automated in vitro selection protocol to obtain RNA-based aptamers: identification of a biostable substance P antagonist
por: Eulberg, Dirk, et al.
Publicado: (2005)

Reflex: intramolecular barcoding of long-range PCR products for sequencing multiple pooled DNAs
por: Casbon, James A., et al.
Publicado: (2013)

Cannot write session to /tmp/vufind_sessions/sess_aqi7ab4hg59ttq45n7bqmpvrk5