Sources of PCR-induced distortions in high-throughput sequencing data sets

PCR permits the exponential and sequence-specific amplification of DNA, even from minute starting quantities. PCR is a fundamental step in preparing DNA samples for high-throughput sequencing. However, there are errors associated with PCR-mediated amplification. Here we examine the effects of four i...

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Detalles Bibliográficos
Autores principales: Kebschull, Justus M., Zador, Anthony M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2015
Materias:
Methods Online
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4666380/
https://www.ncbi.nlm.nih.gov/pubmed/26187991
http://dx.doi.org/10.1093/nar/gkv717

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4666380/
https://www.ncbi.nlm.nih.gov/pubmed/26187991
http://dx.doi.org/10.1093/nar/gkv717

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