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Improvements to a Markerless Allelic Exchange System for Bacillus anthracis

A system was previously developed for conducting I-SceI-mediated allelic exchange in Bacillus anthracis. In this system, recombinational loss of a chromosomally-integrated allelic exchange vector is stimulated by creation of a double-stranded break within the vector by the homing endonuclease I-SceI...

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Detalles Bibliográficos
Autores principales: Plaut, Roger D., Stibitz, Scott
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4666636/
https://www.ncbi.nlm.nih.gov/pubmed/26624016
http://dx.doi.org/10.1371/journal.pone.0142758