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Improvements to a Markerless Allelic Exchange System for Bacillus anthracis
A system was previously developed for conducting I-SceI-mediated allelic exchange in Bacillus anthracis. In this system, recombinational loss of a chromosomally-integrated allelic exchange vector is stimulated by creation of a double-stranded break within the vector by the homing endonuclease I-SceI...
| Autores principales: | , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
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Public Library of Science
2015
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4666636/ https://www.ncbi.nlm.nih.gov/pubmed/26624016 http://dx.doi.org/10.1371/journal.pone.0142758 |